Product nameAnti-SPEF1 antibody
DescriptionRabbit polyclonal to SPEF1
Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Mouse, Human
- Human prostate tissue; Human liver lysate.
General notesThe antibody solution should be gently mixed before use.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151100 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250 - 1/500. Predicted molecular weight: 27 kDa.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Cellular localizationCytoplasm. Cell projection; cilium; flagellum. Cytoplasm; cytoskeleton; cilium axoneme. Note: Present in the tails of developing and epididymal sperm, internal to the fibrous sheath and around the outer dense fibers of the sperm flagellum. Also found at the apical tip of cilia.
- C20orf28 antibody
- Calponin homology and microtubule associated protein antibody
- CLAMP antibody
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling SPEF1 with ab151100 at 1/50 dilution.
All lanes : Anti-SPEF1 antibody (ab151100) at 1/250 dilution
Lane 1 : RT-4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 27 kDa
ab151100 staining SPEF1 in adult mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with PBST (PBS / 0.1% v/v Triton X-100) and blocked with 3% donkey serum / 1% BSA in PBST for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in 1% BSA / 3% donkey serum in PBST) for 48 hours at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
SPEF1 positive staining was detected in some cells in the muscular wall of the tissue. Image shows DAPI counterstain, red field (autofluorescence), antibody staining and overlay.
ab151100 has not yet been referenced specifically in any publications.