1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Cytoplasm. Cell projection; cilium; flagellum. Cytoplasm; cytoskeleton; cilium axoneme. Note: Present in the tails of developing and epididymal sperm, internal to the fibrous sheath and around the outer dense fibers of the sperm flagellum. Also found at the apical tip of cilia.
Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling SPEF1 with ab151100 at 1/50 dilution.
Western blot - Anti-SPEF1 antibody (ab151100)
All lanes : Anti-SPEF1 antibody (ab151100) at 1/250 dilution
Lane 1 : RT-4 cell lysate Lane 2 : U-251 MG cell lysate Lane 3 : Human Plasma lysate Lane 4 : Human Liver lysate Lane 5 : Human Tonsil lysate
Predicted band size: 27 kDa
Immunohistochemistry (Frozen sections) - Anti-SPEF1 antibody (ab151100)This image is courtesy of an anonymous Abreview
ab151100 staining SPEF1 in adult mouse uterus tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with PBST (PBS / 0.1% v/v Triton X-100) and blocked with 3% donkey serum / 1% BSA in PBST for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in 1% BSA / 3% donkey serum in PBST) for 48 hours at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
SPEF1 positive staining was detected in some cells in the muscular wall of the tissue. Image shows DAPI counterstain, red field (autofluorescence), antibody staining and overlay.