Key features and details
- Rabbit polyclonal to SPEN
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPEN antibody
See all SPEN primary antibodies
DescriptionRabbit polyclonal to SPEN
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide from a region between residue 1350 and 1400 of human SPEN, NP_055816.2.
- Whole cell lysate from Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72266 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 µg/ml in enriched samples (ie. immunoprecipitates). Predicted molecular weight: 402 kDa. Not suitable for WB in crude samples.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceSPEN is an essential corepressor protein, which probably regulates different key pathways such as the Notch pathway. It is a negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. SPEN blocks the differentiation of precursor B-cells into marginal zone B-cells. It probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. SPEN may bind both to DNA and RNA.
Cellular localizationNuclear. Associates with chromatin.
- MINT antibody
- Nuclear receptor transcription cofactor antibody
- RBM15C antibody
ab72266 has not yet been referenced specifically in any publications.