Overview

  • Product name
    Anti-Spermidine antibody
  • Description
    Rabbit polyclonal to Spermidine
  • Host species
    Rabbit
  • Specificity
    Reacts with spermidine and cross reacts with spermine.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Immunogen

    Spermidine conjugated to KLH.

Properties

Applications

Our Abpromise guarantee covers the use of ab7318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
WB
  • Application notes
    IHC-P: Use at an assay dependent dilution (PMID 19158080).
    WB: 1/200 - 1/5000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Spermidine is a polyamine compound was originally isolated from semen. It is found ribosomes and living tissues and has various metabolic functions.

    Images

    • ab7318 staining Spermidine in rat lungs by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent fixation in 4% paraformaldehyde overnight and embedded in paraffin, heat mediate antigen retrieval was performed in target retrieval solution and sections were immersed in 3% hydrogen peroxide for 5 min to deplete the endogenous peroxidase activity. Sections were incubated in 1% bovine serum albumin in PBS for 30 min followed by primary antibody at room temperature for 2 h. A primary antibody Enhancer and HRP polymer were used according to the manufacturer's instructions. Left image show staining with ab7318 while the right one show staining with same procedure without primary antibody.

    References

    This product has been referenced in:
    • Yang K  et al. Measuring non-polyaminated lipocalin-2 for cardiometabolic risk assessment. ESC Heart Fail 4:563-575 (2017). Read more (PubMed: 29154418) »
    • Rossi G  et al. Immunohistochemical expression of ornithine decarboxylase, diamine oxidase, putrescine, and spermine in normal canine enterocolic mucosa, in chronic colitis, and in colorectal cancer. Biomed Res Int 2015:172756 (2015). IHC-P . Read more (PubMed: 26550563) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-9 of 9 Abreviews or Q&A

    Answer

    Thank you for contacting us.

    Spermidine and spermine are both polyamines found in living tissues and have various metabolic functions. As they are not proteins, they are not species specific. If the tissue you are interested in probing has spermine or speridine present the antibodies ab7318 and ab26975 should be able to detect the molecules.

    I hope this information has been of help. If you require any further information please do not hesitate to ask.

    Read More

    Answer

    Thank you for contacting us and your interest in our products.

    The two antibodies which you are interested in (ab7318 and ab26975) are both formulations of the whole antiserum. This means that quantification of the exact concentration is not possible as there are several other proteins as well as the active antibody within the preparation. Typically, whole antiserum is of a concentration of 1-10 mg/ml.

    I would suggest optimising the dilution of the antibodies for your experiment, starting with a dilution of 1/50-1/100. From there you should be able to see if you need to reduce or can increase the dilution needed for the optimum results. Ab26975 has not as yet been tested for its use in immunohisotchemistry, we would therefore be very interested in the results you obtain with this antibody. By submitting an abreview for these antibodies you would recieve Abpoints which can be redeemed against the purchase of products from our catalogue, or amazon vouchers. More information on this scheme can be found from the following link:

    www.abcam.com/abreviews

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Answer

    I just wanted to let you know that I am still working on your enquiry. I am still in contact with the originator of this antibody for the information you requested, and am awaiting for an acceptable reply. The originator assures me that there will be a response no later than next Monday. I'm very sorry for the delay. I agree with you that basic information about a product should be available and if not, the product shouldn't be sold. If I don't get an acceptable reply to your question by next Monday, ab7318 will be removed from the Abcam catalog until the problem can be solved. At this time, I request that you fill-out a questionaire regarding the use of ab7318. The questionaire can be found at: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=7318&mode=questionaire This will give us further information regarding the samples you tested and the protocol you used. Once you have submitted the form to us, we will look at the protocol you used and see if there are any suggestions we can make to improve your results. If we determine there is a problem with the antibody and you have purchased it in the last 90 days, we would be happy to replace or refund the antibody for you. I will forward to you the information as soon as I receive it. My apologies for the inconvenience, and thank you for your patience.

    Read More

    Answer

    This antibody reacts with spermidine molecule and there is no differences between spermidine of different species. This antibody cannot be use to identify spermidine synthase.

    Read More

    Answer

    Thank you for your enquiry. As mentioned previously by my colleagues; unfortunately I cannot provide you with further information as this antibody is sourced. The originator of this antibody has not provided details of how the western blot was performed. We do however guarantee that this antibody behaves as detailed on our datasheets by IHC and western blotting. Unfortunately I cannot provide you with references or customers that have applied this antibody as this antibody is yet to be applied in a publication and customer contact details remains confidential information. I am sorry that I cannot be of more assistance on this occasion.

    Read More

    Answer

    This antibody does not cross react with putrescine but it cross reacts with spermine (1:1). We unfortunately do not have any references to published articles using this antibody. The epitope has not been mapped but as the molecule is very short we can't assume that there are multiples sites. I hope this information helps, please do not hesitate to contact us if you need further details.

    Read More

    Answer

    This antibody does not cross react with putrescine but it cross reacts with spermine (1:1). We unfortunately do not have any references to published articles using this antibody. The epitope has not been mapped but as the molecule is very short we can't assume that there are multiples sites. I hope this information helps, please do not hesitate to contact us if you need further details.

    Read More

    Question
    Answer

    Thank you for your email. I have contacted the originator of this antibody to see if they can provide any additional information, and at this time, I am unable to obtain anything further to what we already have on the online datasheet. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

    Read More

    Answer

    Thank you for your email. This particular antibody was originated outside Abcam and we don't have any additional additional information other than what is contained on the online datasheet. This antibody was not characterized and tested in-house, but by the originator. We guarantee all applications and species reactivity stated on the datasheet. I do know that the polyamines (spermidine and spermine) are far too small to be detected by Western blotting. The antibody is used to detect polyamines incorporated in proteins rather than the polyamines themselves.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up