Key features and details
- Rabbit polyclonal to Spermidine
- Suitable for: ELISA, IHC-P, WB
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-Spermidine antibody
DescriptionRabbit polyclonal to Spermidine
SpecificityReacts with spermidine and cross reacts with spermine.
Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
Species reactivityReacts with: Species independent
Spermidine conjugated to KLH.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab7318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/200 - 1/5000.|
RelevanceSpermidine is a polyamine compound was originally isolated from semen. It is found ribosomes and living tissues and has various metabolic functions.
ab7318 staining Spermidine in rat lungs by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Tissue underwent fixation in 4% paraformaldehyde overnight and embedded in paraffin, heat mediate antigen retrieval was performed in target retrieval solution and sections were immersed in 3% hydrogen peroxide for 5 min to deplete the endogenous peroxidase activity. Sections were incubated in 1% bovine serum albumin in PBS for 30 min followed by primary antibody at room temperature for 2 h. A primary antibody Enhancer and HRP polymer were used according to the manufacturer's instructions. Left image show staining with ab7318 while the right one show staining with same procedure without primary antibody.
ab7318 has been referenced in 3 publications.
- Yang K et al. Measuring non-polyaminated lipocalin-2 for cardiometabolic risk assessment. ESC Heart Fail 4:563-575 (2017). PubMed: 29154418
- Rossi G et al. Immunohistochemical expression of ornithine decarboxylase, diamine oxidase, putrescine, and spermine in normal canine enterocolic mucosa, in chronic colitis, and in colorectal cancer. Biomed Res Int 2015:172756 (2015). IHC-P . PubMed: 26550563
- Liao CP et al. Pneumocystis mediates overexpression of antizyme inhibitor resulting in increased polyamine levels and apoptosis in alveolar macrophages. J Biol Chem 284:8174-84 (2009). IHC-P ; Rat . PubMed: 19158080