Spermidine hydrochloride, nNOS inhibitor (ab120057)
Key features and details
- Endogenous polyamine. nNOS inhibitor.
- CAS Number: 334-50-9
- Purity: > 98%
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Spermidine hydrochloride, nNOS inhibitor -
Description
Endogenous polyamine. nNOS inhibitor. -
Biological description
Endogenous polyamine, that binds to the polyamine modulatory site of the NMDA receptor. Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins.
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Purity
> 98% -
CAS Number
334-50-9 -
Chemical structure
Properties
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Chemical name
N-(3-Aminopropyl)butane-1,4-diamine trihydrochloride -
Molecular weight
254.63 -
Molecular formula
C7H19N3.3HCl -
PubChem identifier
9539 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
NCCCNCCCCN.Cl.Cl.Cl -
Source
Synthetic
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Research areas
- Biochemicals
- Pharmacology
- Signaling
- Signal transduction
- Nitric oxide & oxidative stress
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Obesity
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - Spermidine hydrochloride, nNOS inhibitor (ab120057)2D chemical structure image of ab120057, Spermidine hydrochloride, nNOS inhibitor
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Functional Studies - Spermidine hydrochloride, nNOS inhibitor (ab120057)ab9969 staining IL10 in RAW 246.7 cells treated with spermidine hydrochloride (ab120057), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermidine hydrochloride, as described in literature.
The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120057 (spermidine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab120057 has been referenced in 1 publication.
- Ullrich M et al. Bio-orthogonal labeling as a tool to visualize and identify newly synthesized proteins in Caenorhabditis elegans. Nat Protoc 9:2237-55 (2014). PubMed: 25167056