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  1. Link

    spermidine-hydrochloride-nnos-inhibitor-ab120057.pdf

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Spermidine hydrochloride, nNOS inhibitor (ab120057)

  • Datasheet
  • SDS
  • COA
Submit a review Q&A (1)References (1)

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Chemical Structure - Spermidine hydrochloride, nNOS inhibitor (ab120057)
  • Functional Studies - Spermidine hydrochloride, nNOS inhibitor (ab120057)

Key features and details

  • Endogenous polyamine. nNOS inhibitor.
  • CAS Number: 334-50-9
  • Soluble in water to 100 mM
  • Form / State: Solid
  • Source: Synthetic

Overview

  • Product name

    Spermidine hydrochloride, nNOS inhibitor
  • Description

    Endogenous polyamine. nNOS inhibitor.
  • Biological description

    Endogenous polyamine, that binds to the polyamine modulatory site of the NMDA receptor. Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins.

  • CAS Number

    334-50-9
  • Chemical structure

    Chemical Structure

Properties

  • Chemical name

    N-(3-Aminopropyl)butane-1,4-diamine trihydrochloride
  • Molecular weight

    254.63
  • Molecular formula

    C7H19N3.3HCl
  • PubChem identifier

    9539
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in water to 100 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    NCCCNCCCCN.Cl.Cl.Cl
  • Source

    Synthetic

  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Neuroscience
    • Neurotransmission
    • Nitric Oxide
    • NOS
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • AMPA / Kainate
    • Biochemicals
    • Product Range
    • Just Add Water
    • Biochemicals
    • Chemical Type
    • Biochemicals
    • Biochemicals
    • Pharmacology
    • Receptors & Transporters
    • Glutamate
    • NMDA
    • Biochemicals
    • Pharmacology
    • Enzymes
    • Synthetase & synthase
    • Inhibitors
    • Biochemicals
    • Pharmacology
    • Signaling
    • Signal transduction
    • Nitric oxide & oxidative stress
    • NOS
    • Inhibitors
    • Biochemicals
    • Pharmacology
    • Receptors & Transporters
    • Glutamate
    • NMDA
    • Polyamine Site
    • Agonists
    • Biochemicals
    • Research Area
    • Heart disease
    • Signaling
    • Nitric Oxide
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Pain & inflammation
    • Signaling
    • Nitric Oxide
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Addiction
    • Glutamate
    • NMDA
    • Biochemicals
    • Research Area
    • Addiction
    • Glutamate
    • NMDA
    • Polyamine Site
    • Agonists
    • Biochemicals
    • Research Area
    • Alzheimer's Disease
    • Glutamate
    • NMDA
    • Biochemicals
    • Research Area
    • Alzheimer's Disease
    • Glutamate
    • NMDA
    • Polyamine Site
    • Agonists
    • Biochemicals
    • Research Area
    • Alzheimer's Disease
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Alzheimer's Disease
    • Signaling
    • Signal transduction
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Depression
    • Glutamate
    • NMDA
    • Biochemicals
    • Research Area
    • Depression
    • Glutamate
    • NMDA
    • Polyamine Site
    • Agonists
    • Biochemicals
    • Research Area
    • Diabetes
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Diabetes
    • Signaling
    • Signal transduction
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Heart disease
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Hypertension
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Hypertension
    • Signaling
    • Signal transduction
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Obesity
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Obesity
    • Signaling
    • Signal transduction
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Pain & inflammation
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Pain & inflammation
    • Glutamate
    • NMDA
    • Biochemicals
    • Research Area
    • Pain & inflammation
    • Glutamate
    • NMDA
    • Polyamine Site
    • Agonists
    • Biochemicals
    • Research Area
    • Parkinson's Disease
    • Glutamate
    • NMDA
    • Biochemicals
    • Research Area
    • Parkinson's Disease
    • Glutamate
    • NMDA
    • Polyamine Site
    • Agonists
    • Biochemicals
    • Research Area
    • Respiratory disease
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Respiratory disease
    • Signaling
    • Nitric Oxide
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Schizophrenia
    • Glutamate
    • NMDA
    • Biochemicals
    • Research Area
    • Schizophrenia
    • Glutamate
    • NMDA
    • Polyamine Site
    • Agonists
    • Biochemicals
    • Research Area
    • Stroke
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Stroke
    • Signaling
    • Signal transduction
    • Nitric oxide signaling
    • NOS
    • Inhibitors
    • Biochemicals
    • Research Area
    • Stroke
    • Glutamate
    • NMDA
    • Biochemicals
    • Research Area
    • Stroke
    • Glutamate
    • NMDA
    • Polyamine Site
    • Agonists
    • Biochemicals
    • Research Area
    • Cancer
    • Signal transduction
    • Nitric oxide & oxidative stress
    • NOS
    • Inhibitors
    • Neuroscience
    • Processes

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab120057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
Use at an assay dependent concentration.
Notes
Functional Studies
Use at an assay dependent concentration.

Images

  • Chemical Structure - Spermidine hydrochloride, nNOS inhibitor (ab120057)
    Chemical Structure - Spermidine hydrochloride, nNOS inhibitor (ab120057)
    2D chemical structure image of ab120057, Spermidine hydrochloride, nNOS inhibitor
  • Functional Studies - Spermidine hydrochloride, nNOS inhibitor (ab120057)
    Functional Studies - Spermidine hydrochloride, nNOS inhibitor (ab120057)
    ab9969 staining IL10 in RAW 246.7 cells treated with spermidine hydrochloride (ab120057), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermidine hydrochloride, as described in literature.
    The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120057 (spermidine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download
  • COA

References (1)

Publishing research using ab120057? Please let us know so that we can cite the reference in this datasheet.

ab120057 has been referenced in 1 publication.

  • Ullrich M  et al. Bio-orthogonal labeling as a tool to visualize and identify newly synthesized proteins in Caenorhabditis elegans. Nat Protoc 9:2237-55 (2014). PubMed: 25167056

Customer reviews and Q&As

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Submit a review Submit a question

Question

I’m interested in two ingredients of your portfolio: spermine and spermidine. Could -you, please, send me for each ingredient the following information:

-Technical data sheet

- Recommended use (cosmetic, dietary, pharmaceutical)

- Recommended dosage

-Price/ Kg and minimum order

-Regulatory requirements

-Origin (synthetic or natural)

-Method of dosage

Thank you in advance

Best regards

Read More

Abcam community

Verified customer

Asked on Oct 18 2012

Answer

Thank you for contacting us.

- The datasheets can be downloaded from the links;

https://www.abcam.com/Spermine-ab120051.html

https://www.abcam.com/Spermidine-hydrochloride-ab120057.html

- These products are for research use only and are not intended for diagnostic of therapeutic use.

- ab120057 will cost Euro 55 per 100mg and ab120051 will cost Euro 55 for 1gram.

- These are synthetic chemicals

These chemicals are for research use only.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
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Read More

Abcam Scientific Support

Answered on Oct 18 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

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