Overview

  • Product name
    Spermidine hydrochloride, nNOS inhibitor
  • Description
    Endogenous polyamine. nNOS inhibitor.
  • Biological description
    Endogenous polyamine, that binds to the polyamine modulatory site of the NMDA receptor. Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins.
  • Purity
    > 98%
  • CAS Number
    334-50-9
  • Chemical structure
    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab120057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab9969 staining IL10 in RAW 246.7 cells treated with spermidine hydrochloride (ab120057), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermidine hydrochloride, as described in literature.
    The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120057 (spermidine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

- The datasheets can be downloaded from the links;

https://www.abcam.com/Spermine-ab120051.html

https://www.abcam.com/Spermidine-hydrochloride-ab120057.html

- These products are for research use only and are not intended for diagnostic of therapeutic use.

- ab120057 will cost Euro 55 per 100mg and ab120051 will cost Euro 55 for 1gram.

- These are synthetic chemicals

These chemicals are for research use only.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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