Spermidine hydrochloride, nNOS inhibitor (ab120057)
- Datasheet
- References (1)
Overview
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Product nameSpermidine hydrochloride, nNOS inhibitor
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DescriptionEndogenous polyamine. nNOS inhibitor.
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Biological descriptionEndogenous polyamine, that binds to the polyamine modulatory site of the NMDA receptor. Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins.
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Purity> 98%
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CAS Number334-50-9
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Chemical structure
Properties
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Chemical nameN-(3-Aminopropyl)butane-1,4-diamine trihydrochloride
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Molecular weight254.63
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Molecular formulaC7H19N3.3HCl
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PubChem identifier9539
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Storage instructionsStore at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
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Solubility overviewSoluble in water to 100 mM
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Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILESNCCCNCCCCN.Cl.Cl.Cl
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Source
Synthetic
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Research areas
- Biochemicals
- Pharmacology
- Signaling
- Signal transduction
- Nitric oxide & oxidative stress
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Obesity
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
Applications
Our Abpromise guarantee covers the use of ab120057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
Images
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Functional Studies - Spermidine hydrochloride, nNOS inhibitor (ab120057)ab9969 staining IL10 in RAW 246.7 cells treated with spermidine hydrochloride (ab120057), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermidine hydrochloride, as described in literature.
The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120057 (spermidine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
References
This product has been referenced in:
- Baumann P et al. Purification of human Rad51 protein by selective spermidine precipitation. Mutat Res 384:65-72 (1997). Read more (PubMed: 9298115) »
- Hu J et al. Polyamines inhibit nitric oxide synthase in rat cerebellum. Neurosci Lett 175:41-5 (1994). Read more (PubMed: 7526294) »
- Williams K et al. Sensitivity of the N-methyl-D-aspartate receptor to polyamines is controlled by NR2 subunits. Mol Pharmacol 45:803-9 (1994). Read more (PubMed: 8190097) »
