Anti-Spermine synthase antibody (ab101458)

Overview

  • Product name
    Anti-Spermine synthase antibody
    See all Spermine synthase primary antibodies
  • Description
    Rabbit polyclonal to Spermine synthase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 144-336 of Human Spermine synthase (AAH09898).

  • Positive control
    • 293T, A431 and Jurkat whole cell lysates; Raji cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab101458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 41 kDa.

Target

  • Function
    Required for normal viability, growth and fertility.
  • Pathway
    Amine and polyamine biosynthesis; spermine biosynthesis; spermine from spermidine: step 1/1.
  • Involvement in disease
    Defects in SMS are the cause of Snyder-Robinson syndrome (SRS) [MIM:309583]; also known as X-linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive.
  • Sequence similarities
    Belongs to the spermidine/spermine synthase family.
  • Domain
    Composed of 3 domains: the N-terminal domain has structural similarity to S-adenosylmethionine decarboxylase, the central domain is made up of four beta strands and the C-terminal domain is similar in structure to spermidine synthase. The N- and C-terminal domains are both required for activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • MRSR antibody
    • SMS antibody
    • Snyder Robinson X linked mental retardation syndrome antibody
    • Spermidine aminopropyltransferase antibody
    • Spermine synthase antibody
    • SPMSY antibody
    • SpS antibody
    • SPSY_HUMAN antibody
    • SRS antibody
    see all

Images

  • All lanes : Anti-Spermine synthase antibody (ab101458) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 41 kDa



    10% SDS-PAGE

References

ab101458 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Retina)
Gel Running Conditions
Reduced Denaturing (10% Gel)
Loading amount
20 µg
Specification
Retina
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

David S. Rios Otero

Verified customer

Submitted Apr 27 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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