Key features and details
- Rabbit polyclonal to Spermine synthase
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Spermine synthase antibody
See all Spermine synthase primary antibodies
DescriptionRabbit polyclonal to Spermine synthase
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Spermine synthase aa 16-191.
Database link: P52788
- IHC-P: Human ovarian and prostate cancer tissues. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab222836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
FunctionRequired for normal viability, growth and fertility.
PathwayAmine and polyamine biosynthesis; spermine biosynthesis; spermine from spermidine: step 1/1.
Involvement in diseaseDefects in SMS are the cause of Snyder-Robinson syndrome (SRS) [MIM:309583]; also known as X-linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive.
Sequence similaritiesBelongs to the spermidine/spermine synthase family.
DomainComposed of 3 domains: the N-terminal domain has structural similarity to S-adenosylmethionine decarboxylase, the central domain is made up of four beta strands and the C-terminal domain is similar in structure to spermidine synthase. The N- and C-terminal domains are both required for activity.
- Information by UniProt
- MRSR antibody
- SMS antibody
- Snyder Robinson X linked mental retardation syndrome antibody
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Spermine synthase (green) using ab222836 at 1/100 dilution in ICC/IF. Alexa Fluor 488-conjugated Goat anti-Rabbit IgG (H+L) was used as secondary antibody.
Paraffin-embedded human prostate cancer tissue stained for Spermine synthase using ab222836 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human ovarian cancer tissue stained for Spermine synthase using ab222836 at 1/100 dilution in immunohistochemical analysis.
ab222836 has not yet been referenced specifically in any publications.