Product nameAnti-Spermine synthase antibody [EPR9252(B)]
See all Spermine synthase primary antibodies
DescriptionRabbit monoclonal [EPR9252(B)] to Spermine synthase
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Spermine synthase. The exact sequence is proprietary.
- Recombinant Human Spermine synthase protein (ab116202) can be used as a positive control in WB. Human fetal heart extract, HeLa cell extract, Jurkat cell extract, K562 cell extract
Storage instructionsShipped at 4°C. Store at -20°C.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab156879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 41 kDa.|
|ICC/IF||1/250 - 1/500.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRequired for normal viability, growth and fertility.
PathwayAmine and polyamine biosynthesis; spermine biosynthesis; spermine from spermidine: step 1/1.
Involvement in diseaseDefects in SMS are the cause of Snyder-Robinson syndrome (SRS) [MIM:309583]; also known as X-linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive.
Sequence similaritiesBelongs to the spermidine/spermine synthase family.
DomainComposed of 3 domains: the N-terminal domain has structural similarity to S-adenosylmethionine decarboxylase, the central domain is made up of four beta strands and the C-terminal domain is similar in structure to spermidine synthase. The N- and C-terminal domains are both required for activity.
- Information by UniProt
- MRSR antibody
- SMS antibody
- Snyder Robinson X linked mental retardation syndrome antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Spermine synthase knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab156879 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab156879 was shown to specifically react with Spermine synthase when Spermine synthase knockout samples were used. Wild-type and Spermine synthase knockout samples were subjected to SDS-PAGE. ab156879 and ab18058 (loading control to vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes : Anti-Spermine synthase antibody [EPR9252(B)] (ab156879) at 1/1000 dilution
Lane 1 : Human fetal heart extract
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDa
Immunofluorescence analysis of HeLa cells labeling Spermine Synthase with ab156879 antibody at a dilution of 1/250.
This product has been referenced in:
- Gong S et al. Placental polyamine metabolism differs by fetal sex, fetal growth restriction, and preeclampsia. JCI Insight 3:N/A (2018). Read more (PubMed: 29997303) »
- Bistulfi G et al. The essential role of methylthioadenosine phosphorylase in prostate cancer. Oncotarget 7:14380-93 (2016). WB . Read more (PubMed: 26910893) »