Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)

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Overview

  • Product name

    Anti-Spermine synthase antibody [EPR9252(B)]
    See all Spermine synthase primary antibodies

  • Description

    Rabbit monoclonal [EPR9252(B)] to Spermine synthase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Spermine synthase. The exact sequence is proprietary.

  • Positive control

    • Recombinant Human Spermine synthase protein (ab116202) can be used as a positive control in WB. Human fetal heart extract, HeLa cell extract, Jurkat cell extract, K562 cell extract

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Required for normal viability, growth and fertility.

    • Pathway

      Amine and polyamine biosynthesis; spermine biosynthesis; spermine from spermidine: step 1/1.

    • Involvement in disease

      Defects in SMS are the cause of Snyder-Robinson syndrome (SRS) [MIM:309583]; also known as X-linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive.

    • Sequence similarities

      Belongs to the spermidine/spermine synthase family.

    • Domain

      Composed of 3 domains: the N-terminal domain has structural similarity to S-adenosylmethionine decarboxylase, the central domain is made up of four beta strands and the C-terminal domain is similar in structure to spermidine synthase. The N- and C-terminal domains are both required for activity.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • MRSR antibody
      • SMS antibody
      • Snyder Robinson X linked mental retardation syndrome antibody
      • Spermidine aminopropyltransferase antibody
      • Spermine synthase antibody
      • SPMSY antibody
      • SpS antibody
      • SPSY_HUMAN antibody
      • SRS antibody
      see all

    Images

    • Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
      Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Spermine synthase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab156879 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.
      ab156879 was shown to specifically react with Spermine synthase when Spermine synthase knockout samples were used. Wild-type and Spermine synthase knockout samples were subjected to SDS-PAGE. ab156879 and ab18058 (loading control to vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
      Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
      All lanes : Anti-Spermine synthase antibody [EPR9252(B)] (ab156879) at 1/1000 dilution

      Lane 1 : Human fetal heart extract
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 41 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
      Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
      Immunofluorescence analysis of HeLa cells labeling Spermine Synthase with ab156879 antibody at a dilution of 1/250.

    References

    This product has been referenced in:

    • Gong S  et al. Placental polyamine metabolism differs by fetal sex, fetal growth restriction, and preeclampsia. JCI Insight 3:N/A (2018). Read more (PubMed: 29997303) »
    • Bistulfi G  et al. The essential role of methylthioadenosine phosphorylase in prostate cancer. Oncotarget 7:14380-93 (2016). WB . Read more (PubMed: 26910893) »
    See all 2 Publications for this product

    Publishing research using ab156879? Please let us know so that we can cite the reference in this datasheet.

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