Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)

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Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
  • Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
  • Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
  • Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9252(B)] to Spermine synthase
  • Suitable for: WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Spermine synthase antibody [EPR9252(B)]
    See all Spermine synthase primary antibodies

  • Description

    Rabbit monoclonal [EPR9252(B)] to Spermine synthase

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Spermine synthase aa 100-200. The exact sequence is proprietary.
    Database link: P52788

  • Positive control

    • Recombinant Human Spermine synthase protein (ab116202) can be used as a positive control in WB. Human fetal heart extract, HeLa cell extract, Jurkat cell extract, K562 cell extract

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
ICC/IF
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
ICC/IF
1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Required for normal viability, growth and fertility.

    • Pathway

      Amine and polyamine biosynthesis; spermine biosynthesis; spermine from spermidine: step 1/1.

    • Involvement in disease

      Defects in SMS are the cause of Snyder-Robinson syndrome (SRS) [MIM:309583]; also known as X-linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive.

    • Sequence similarities

      Belongs to the spermidine/spermine synthase family.

    • Domain

      Composed of 3 domains: the N-terminal domain has structural similarity to S-adenosylmethionine decarboxylase, the central domain is made up of four beta strands and the C-terminal domain is similar in structure to spermidine synthase. The N- and C-terminal domains are both required for activity.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • MRSR antibody
      • SMS antibody
      • Snyder Robinson X linked mental retardation syndrome antibody
      • Spermidine aminopropyltransferase antibody
      • Spermine synthase antibody
      • SPMSY antibody
      • SpS antibody
      • SPSY_HUMAN antibody
      • SRS antibody
      see all

    Images

    • Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
      Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Spermine synthase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab156879 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.
      ab156879 was shown to specifically react with Spermine synthase when Spermine synthase knockout samples were used. Wild-type and Spermine synthase knockout samples were subjected to SDS-PAGE. ab156879 and ab18058 (loading control to vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
      Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
      All lanes : Anti-Spermine synthase antibody [EPR9252(B)] (ab156879) at 1/1000 dilution

      Lane 1 : Human fetal heart extract
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 41 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
      Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
      Immunofluorescence analysis of HeLa cells labeling Spermine Synthase with ab156879 antibody at a dilution of 1/250.
    • Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
      Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)

    References (3)

    Publishing research using ab156879? Please let us know so that we can cite the reference in this datasheet.

    ab156879 has been referenced in 3 publications.

    • Chen J  et al. Supramolecular trap for catching polyamines in cells as an anti-tumor strategy. Nat Commun 10:3546 (2019). PubMed: 31391464
    • Gong S  et al. Placental polyamine metabolism differs by fetal sex, fetal growth restriction, and preeclampsia. JCI Insight 3:N/A (2018). PubMed: 29997303
    • Bistulfi G  et al. The essential role of methylthioadenosine phosphorylase in prostate cancer. Oncotarget 7:14380-93 (2016). WB . PubMed: 26910893

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