Recombinant Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-Spermine synthase antibody [EPR9252(B)]
See all Spermine synthase primary antibodies -
Description
Rabbit monoclonal [EPR9252(B)] to Spermine synthase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Spermine synthase. The exact sequence is proprietary.
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Positive control
- Recombinant Human Spermine synthase protein (ab116202) can be used as a positive control in WB. Human fetal heart extract, HeLa cell extract, Jurkat cell extract, K562 cell extract
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9252(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab156879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 41 kDa. | |
| ICC/IF | 1/250 - 1/500. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Required for normal viability, growth and fertility. -
Pathway
Amine and polyamine biosynthesis; spermine biosynthesis; spermine from spermidine: step 1/1. -
Involvement in disease
Defects in SMS are the cause of Snyder-Robinson syndrome (SRS) [MIM:309583]; also known as X-linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive. -
Sequence similarities
Belongs to the spermidine/spermine synthase family. -
Domain
Composed of 3 domains: the N-terminal domain has structural similarity to S-adenosylmethionine decarboxylase, the central domain is made up of four beta strands and the C-terminal domain is similar in structure to spermidine synthase. The N- and C-terminal domains are both required for activity. - Information by UniProt
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Database links
- Entrez Gene: 6611 Human
- Entrez Gene: 20603 Mouse
- Entrez Gene: 671878 Mouse
- Entrez Gene: 363469 Rat
- Omim: 300105 Human
- SwissProt: P52788 Human
- SwissProt: P97355 Mouse
- Unigene: 727552 Human
see all -
Alternative names
- MRSR antibody
- SMS antibody
- Snyder Robinson X linked mental retardation syndrome antibody
see all
Images
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![Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)](https://www.abcam.com/ps/products/156/ab156879/Images/ab156879-266947-anti-spermine-synthase-antibody-epr9252b-western-blot.jpg)
Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Spermine synthase knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab156879 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab156879 was shown to specifically react with Spermine synthase when Spermine synthase knockout samples were used. Wild-type and Spermine synthase knockout samples were subjected to SDS-PAGE. ab156879 and ab18058 (loading control to vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)](https://www.abcam.com/ps/products/156/ab156879/Images/ab156879-164077-anti-spermine-synthase-antibody-epr9252b-western-blot.jpg)
Western blot - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)All lanes : Anti-Spermine synthase antibody [EPR9252(B)] (ab156879) at 1/1000 dilution
Lane 1 : Human fetal heart extract
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)](https://www.abcam.com/ps/products/156/ab156879/Images/ab156879-164076-anti-spermine-synthase-antibody-epr9252b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)Immunofluorescence analysis of HeLa cells labeling Spermine Synthase with ab156879 antibody at a dilution of 1/250.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Gong S et al. Placental polyamine metabolism differs by fetal sex, fetal growth restriction, and preeclampsia. JCI Insight 3:N/A (2018). Read more (PubMed: 29997303) »
- Bistulfi G et al. The essential role of methylthioadenosine phosphorylase in prostate cancer. Oncotarget 7:14380-93 (2016). WB . Read more (PubMed: 26910893) »
