Recombinant Anti-Spermine synthase antibody [EPR9252(B)] (ab156879)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9252(B)] to Spermine synthase
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Spermine synthase antibody [EPR9252(B)]
See all Spermine synthase primary antibodies -
Description
Rabbit monoclonal [EPR9252(B)] to Spermine synthase -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanWB Human -
Immunogen
Synthetic peptide within Human Spermine synthase aa 100-200. The exact sequence is proprietary.
Database link: P52788 -
Positive control
- Recombinant Human Spermine synthase protein (ab116202) can be used as a positive control in WB. Human fetal heart extract, HeLa cell extract, Jurkat cell extract, K562 cell extract
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9252(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC/IF |
Human
|
WB |
Human
|
All applications |
Rat
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
|
|
ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 41 kDa. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Required for normal viability, growth and fertility. -
Pathway
Amine and polyamine biosynthesis; spermine biosynthesis; spermine from spermidine: step 1/1. -
Involvement in disease
Defects in SMS are the cause of Snyder-Robinson syndrome (SRS) [MIM:309583]; also known as X-linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive. -
Sequence similarities
Belongs to the spermidine/spermine synthase family. -
Domain
Composed of 3 domains: the N-terminal domain has structural similarity to S-adenosylmethionine decarboxylase, the central domain is made up of four beta strands and the C-terminal domain is similar in structure to spermidine synthase. The N- and C-terminal domains are both required for activity. - Information by UniProt
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Database links
- Entrez Gene: 6611 Human
- Entrez Gene: 20603 Mouse
- Entrez Gene: 671878 Mouse
- Entrez Gene: 363469 Rat
- Omim: 300105 Human
- SwissProt: P52788 Human
- SwissProt: P97355 Mouse
- Unigene: 727552 Human
see all -
Alternative names
- MRSR antibody
- SMS antibody
- Snyder Robinson X linked mental retardation syndrome antibody
see all
Images
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Spermine synthase knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab156879 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab156879 was shown to specifically react with Spermine synthase when Spermine synthase knockout samples were used. Wild-type and Spermine synthase knockout samples were subjected to SDS-PAGE. ab156879 and ab18058 (loading control to vinculin) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-Spermine synthase antibody [EPR9252(B)] (ab156879) at 1/1000 dilution
Lane 1 : Human fetal heart extract
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDa -
Immunofluorescence analysis of HeLa cells labeling Spermine Synthase with ab156879 antibody at a dilution of 1/250.
Protocols
Datasheets and documents
References (3)
ab156879 has been referenced in 3 publications.
- Chen J et al. Supramolecular trap for catching polyamines in cells as an anti-tumor strategy. Nat Commun 10:3546 (2019). PubMed: 31391464
- Gong S et al. Placental polyamine metabolism differs by fetal sex, fetal growth restriction, and preeclampsia. JCI Insight 3:N/A (2018). PubMed: 29997303
- Bistulfi G et al. The essential role of methylthioadenosine phosphorylase in prostate cancer. Oncotarget 7:14380-93 (2016). WB . PubMed: 26910893