Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-Spermine synthase antibody [EPR9253] (ab151547)

Overview

  • Product name

    Anti-Spermine synthase antibody [EPR9253]
    See all Spermine synthase primary antibodies
  • Description

    Rabbit monoclonal [EPR9253] to Spermine synthase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Spermine synthase aa 300-400. The exact sequence is proprietary.

  • Positive control

    • Human fetal heart, HeLa, Jurkat and K562 lysates; HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 41 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Required for normal viability, growth and fertility.
    • Pathway

      Amine and polyamine biosynthesis; spermine biosynthesis; spermine from spermidine: step 1/1.
    • Involvement in disease

      Defects in SMS are the cause of Snyder-Robinson syndrome (SRS) [MIM:309583]; also known as X-linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive.
    • Sequence similarities

      Belongs to the spermidine/spermine synthase family.
    • Domain

      Composed of 3 domains: the N-terminal domain has structural similarity to S-adenosylmethionine decarboxylase, the central domain is made up of four beta strands and the C-terminal domain is similar in structure to spermidine synthase. The N- and C-terminal domains are both required for activity.
    • Information by UniProt
    • Database links

    • Alternative names

      • MRSR antibody
      • SMS antibody
      • Snyder Robinson X linked mental retardation syndrome antibody
      • Spermidine aminopropyltransferase antibody
      • Spermine synthase antibody
      • SPMSY antibody
      • SpS antibody
      • SPSY_HUMAN antibody
      • SRS antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Spermine synthase knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab151547 observed at 40 kDa. Red - loading control, ab18058, observed at 124 kDa.

      ab151547 was shown to specifically react with Spermine synthase when Spermine synthase knockout samples were used. Wild-type and Spermine synthase knockout samples were subjected to SDS-PAGE. ab151547 and ab18058 (loading control to vinculin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Spermine synthase antibody [EPR9253] (ab151547) at 1/1000 dilution

      Lane 1 : Human fetal heart lysate
      Lane 2 : HeLa lysate
      Lane 3 : Jurkat lysate
      Lane 4 : K562 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 41 kDa

    • Flow cytometric analysis of permeabilized HeLa cells labeling Spermine synthase (red) with ab151547 at 1/10 dilution, compared to a nonspecific control antibody (green).

    References

    ab151547 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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