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    spexyte-intracellular-ph-calibration-buffer-kit-ab284662.pdf

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Spexyte™ Intracellular pH Calibration Buffer Kit (ab284662)

  • Datasheet
  • SDS
  • Protocol Booklet
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Standard curve created using BCFL, AM with Spexyte™ Intracellular pH Calibration Buffer Kit (ab284662)

    Key features and details

    • Assay type: Quantitative
    • Detection method: Fluorescent

    Overview

    • Product name

      Spexyte™ Intracellular pH Calibration Buffer Kit
      See all Intracellular pH kits
    • Detection method

      Fluorescent
    • Assay type

      Quantitative
    • Product overview

      Spexyte™ Intracellular pH Calibration Buffer Kit (ab284662) provides a range of pH calibration buffers (pH 4.5~ 8.0) with nigericin, which modulate the intracellular pH with the external pH in the presence of 100"150 mM K+. When used in conjunction with pH indicators, such as BCFL, AM or BCECF, AM, ab284662 can create a standard curve which is used to determine the intracellular pH.

    • Notes

      Intracellular pH (pHi) plays an important modulating role in many cellular events, including cell volume regulation, cellular metabolism, calcium regulation, receptor-mediated signal transduction, ion transport, endocytosis, and other cellular processes. Intracellular pH is generally 6.8 ~7.4 in the cytosol and 4.5~6.0 in the acidic organelles. Intracellular pH changes have significant physiological effects, e.g., the pH-dependent concentration of intracellular messengers such as Ca2+ and cAMP affects cellular signaling. Several recent reports showed the dysregulated pH is emerging as a hallmark of cancer cells.

    Properties

    • Storage instructions

      Store at +4°C. Please refer to protocols.
    • Components 100 tests
      DMSO 1 x 300µl
      Nigericin, free acid 1 x 2mg
      pH=4.5 Buffer 1 x 10ml
      pH=5 Buffer 1 x 10ml
      pH=5.5 Buffer 1 x 10ml
      pH=6 Buffer 1 x 10ml
      pH=6.5 Buffer 1 x 10ml
      pH=7 Buffer 1 x 10ml
      pH=7.5 Buffer 1 x 10ml
      pH=8 Buffer 1 x 10ml

    Images

    • Standard curve created using BCFL, AM with Spexyte™ Intracellular pH Calibration Buffer Kit (ab284662)
      Standard curve created using BCFL, AM with Spexyte™ Intracellular pH Calibration Buffer Kit (ab284662)

      Standard curve created using BCFL, AM with Spexyte™ Intracellular pH Calibration Buffer Kit (ab284662). HeLa cells were incubated with 5µM BCFL, AM for 30 minutes at room temperature. The Intracellular pH Calibration Buffer Kit was used to clamp the intracellular pH with extracellular buffers at pH 4.5 to 8.0. Intracellular pH vs. relative fluorescence ratio of Ex/Em= 440/ 530 nm and 500 nm/530 nm were plotted and a 4-parameter trendline was fitted to get the pH standard curve.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

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    References (0)

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