Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
Product nameSpexyte™ Intracellular pH Calibration Buffer Kit
See all Intracellular pH kits
Spexyte™ Intracellular pH Calibration Buffer Kit (ab284662) provides a range of pH calibration buffers (pH 4.5~ 8.0) with nigericin, which modulate the intracellular pH with the external pH in the presence of 100"150 mM K+. When used in conjunction with pH indicators, such as BCFL, AM or BCECF, AM, ab284662 can create a standard curve which is used to determine the intracellular pH.
Intracellular pH (pHi) plays an important modulating role in many cellular events, including cell volume regulation, cellular metabolism, calcium regulation, receptor-mediated signal transduction, ion transport, endocytosis, and other cellular processes. Intracellular pH is generally 6.8 ~7.4 in the cytosol and 4.5~6.0 in the acidic organelles. Intracellular pH changes have significant physiological effects, e.g., the pH-dependent concentration of intracellular messengers such as Ca2+ and cAMP affects cellular signaling. Several recent reports showed the dysregulated pH is emerging as a hallmark of cancer cells.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 100 tests DMSO 1 x 300µl Nigericin, free acid 1 x 2mg pH=4.5 Buffer 1 x 10ml pH=5 Buffer 1 x 10ml pH=5.5 Buffer 1 x 10ml pH=6 Buffer 1 x 10ml pH=6.5 Buffer 1 x 10ml pH=7 Buffer 1 x 10ml pH=7.5 Buffer 1 x 10ml pH=8 Buffer 1 x 10ml
Standard curve created using BCFL, AM with Spexyte™ Intracellular pH Calibration Buffer Kit (ab284662). HeLa cells were incubated with 5µM BCFL, AM for 30 minutes at room temperature. The Intracellular pH Calibration Buffer Kit was used to clamp the intracellular pH with extracellular buffers at pH 4.5 to 8.0. Intracellular pH vs. relative fluorescence ratio of Ex/Em= 440/ 530 nm and 500 nm/530 nm were plotted and a 4-parameter trendline was fitted to get the pH standard curve.
ab284662 has not yet been referenced specifically in any publications.