Anti-SPF45 antibody (ab101441)
Key features and details
- Rabbit polyclonal to SPF45
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPF45 antibody
See all SPF45 primary antibodies -
Description
Rabbit polyclonal to SPF45 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human SPF45 aa 227-370. (AAH07871)
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Positive control
- 293T and Raji cell lines
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General notes
This product was previously labelled as RBM17
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab101441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
Target
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Function
Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia. -
Sequence similarities
Contains 1 G-patch domain.
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 84991 Human
- Entrez Gene: 76938 Mouse
- Entrez Gene: 291295 Rat
- Omim: 606935 Human
- SwissProt: Q96I25 Human
- SwissProt: Q8JZX4 Mouse
- Unigene: 498548 Human
- Unigene: 182769 Mouse
see all -
Alternative names
- 45 kDa-splicing factor antibody
- RBM 17 antibody
- Rbm17 antibody
see all
Images
References (1)
ab101441 has been referenced in 1 publication.
- Han Y et al. Downregulation of RNA binding motif protein 17 expression inhibits proliferation of hypopharyngeal carcinoma FaDu cells. Oncol Lett 15:5680-5684 (2018). PubMed: 29552202