Anti-SPF45 antibody (ab101441)

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Overview

  • Product name

    Anti-SPF45 antibody
    See all SPF45 primary antibodies

  • Description

    Rabbit polyclonal to SPF45

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Recombinant fragment corresponding to Human SPF45 aa 227-370. (AAH07871)

  • Positive control

    • 293T and Raji cell lines

  • General notes

     This product was previously labelled as RBM17

     

Properties

Applications

Our Abpromise guarantee covers the use of ab101441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.

Target

  • Function

    Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.

  • Sequence similarities

    Contains 1 G-patch domain.
    Contains 1 RRM (RNA recognition motif) domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 45 kDa-splicing factor antibody
    • RBM 17 antibody
    • Rbm17 antibody
    • RNA binding motif protein 17 antibody
    • RNA-binding motif protein 17 antibody
    • SPF 45 antibody
    • SPF45 antibody
    • SPF45_HUMAN antibody
    • Splicing factor (45 kD) antibody
    • Splicing factor (45kD) antibody
    • Splicing factor 45 antibody
    • Splicing factor 45 kDa antibody
    • Splicing factor 45kDa antibody
    see all

Images

  • Western blot - Anti-SPF45 antibody (ab101441)
    Western blot - Anti-SPF45 antibody (ab101441)
    Anti-SPF45 antibody (ab101441) at 1/500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 45 kDa



    10% SDS PAGE

References

This product has been referenced in:

  • Han Y  et al. Downregulation of RNA binding motif protein 17 expression inhibits proliferation of hypopharyngeal carcinoma FaDu cells. Oncol Lett 15:5680-5684 (2018). Read more (PubMed: 29552202) »
See 1 Publication for this product

Publishing research using ab101441? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Multiple bands detected in blots of mouse brain cell lysates.

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Answer

The antibody has not been tested on mouse samples before, so there may be an issue with low cross-reactivity with mouse RBM17, though reactivity is predicted based on homology with the immunogen. In any case, the high background is the main issue.

If the new lysate you are preparing, and/or the switch we discussed to milk from BSA for blocking/diluting does not help, you may want to consider preparing samples from isolated nuclei, to enrich for the protein. Detection may be difficult with any antibody against this target, if the protein is scarce. Another approach is to immunoprecipitate it, for instance with the antibody I mentioned, ab114968, though it has not been tested against mouse protein either.

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Question

Re your RBM17 antibody (#ab101441), can you tell me why it picks up an ˜70kda band in addition to the ˜54 and 45kda bands (which correspond with its molecular weight)?

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Answer


In my opinion, the 54 kDa band is phosphorylated RBM17 and the lower 45 kDa band is the dephosphorylated version. The 70 kDa band appears to be a non-specific band.

I came to these conclusions looking at the western blots for two other anti-RBM17 antibodies:
1. https://www.abcam.com/rbm17-antibody-ab114968.html
2. https://www.abcam.com/rbm17-antibody-ab169132.html

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