Anti-SPF45 antibody (ab101441)

Submit a review Q&A (2)References (1)
Western blot - Anti-SPF45 antibody (ab101441)

    Key features and details

    • Rabbit polyclonal to SPF45
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-SPF45 antibody
      See all SPF45 primary antibodies

    • Description

      Rabbit polyclonal to SPF45

    • Host species

      Rabbit

    • Tested applications

      Suitable for: WBmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Cow

    • Immunogen

      Recombinant fragment corresponding to Human SPF45 aa 227-370. (AAH07871)

    • Positive control

      • 293T and Raji cell lines

    • General notes

       This product was previously labelled as RBM17

       

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    Applications

    Our Abpromise guarantee covers the use of ab101441 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/3000. Predicted molecular weight: 45 kDa.

    Target

    • Function

      Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.

    • Sequence similarities

      Contains 1 G-patch domain.
      Contains 1 RRM (RNA recognition motif) domain.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • 45 kDa-splicing factor antibody
      • RBM 17 antibody
      • Rbm17 antibody
      • RNA binding motif protein 17 antibody
      • RNA-binding motif protein 17 antibody
      • SPF 45 antibody
      • SPF45 antibody
      • SPF45_HUMAN antibody
      • Splicing factor (45 kD) antibody
      • Splicing factor (45kD) antibody
      • Splicing factor 45 antibody
      • Splicing factor 45 kDa antibody
      • Splicing factor 45kDa antibody
      see all

    Images

    • Western blot - Anti-SPF45 antibody (ab101441)
      Western blot - Anti-SPF45 antibody (ab101441)
      Anti-SPF45 antibody (ab101441) at 1/500 dilution + Raji whole cell lysate at 30 µg

      Predicted band size: 45 kDa



      10% SDS PAGE

    References (1)

    Publishing research using ab101441? Please let us know so that we can cite the reference in this datasheet.

    ab101441 has been referenced in 1 publication.

    • Han Y  et al. Downregulation of RNA binding motif protein 17 expression inhibits proliferation of hypopharyngeal carcinoma FaDu cells. Oncol Lett 15:5680-5684 (2018). PubMed: 29552202

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Multiple bands detected in blots of mouse brain cell lysates.

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    Answer

    The antibody has not been tested on mouse samples before, so there may be an issue with low cross-reactivity with mouse RBM17, though reactivity is predicted based on homology with the immunogen. In any case, the high background is the main issue.

    If the new lysate you are preparing, and/or the switch we discussed to milk from BSA for blocking/diluting does not help, you may want to consider preparing samples from isolated nuclei, to enrich for the protein. Detection may be difficult with any antibody against this target, if the protein is scarce. Another approach is to immunoprecipitate it, for instance with the antibody I mentioned, ab114968, though it has not been tested against mouse protein either.

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    Question

    Re your RBM17 antibody (#ab101441), can you tell me why it picks up an ˜70kda band in addition to the ˜54 and 45kda bands (which correspond with its molecular weight)?

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    Answer


    In my opinion, the 54 kDa band is phosphorylated RBM17 and the lower 45 kDa band is the dephosphorylated version. The 70 kDa band appears to be a non-specific band.

    I came to these conclusions looking at the western blots for two other anti-RBM17 antibodies:
    1. https://www.abcam.com/rbm17-antibody-ab114968.html
    2. https://www.abcam.com/rbm17-antibody-ab169132.html

    Read More

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