Anti-SPF45 antibody (ab114968)
Key features and details
- Rabbit polyclonal to SPF45
- Suitable for: IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SPF45 antibody
See all SPF45 primary antibodies -
Description
Rabbit polyclonal to SPF45 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide corresponding to Human SPF45 aa 25-75.
Database link: NP_116294.1 -
Positive control
- WB: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue, mouse plasmacytoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab114968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia. -
Sequence similarities
Contains 1 G-patch domain.
Contains 1 RRM (RNA recognition motif) domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 84991 Human
- Entrez Gene: 76938 Mouse
- Entrez Gene: 291295 Rat
- Omim: 606935 Human
- SwissProt: Q96I25 Human
- SwissProt: Q8JZX4 Mouse
- Unigene: 498548 Human
- Unigene: 182769 Mouse
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Alternative names
- 45 kDa-splicing factor antibody
- RBM 17 antibody
- Rbm17 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling SPF45 with ab114968 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of SPF45 by Western Blot of Immunprecipitate.
anti-SPF45 at 0.4µg/ml staining SPF45 in HeLa whole cell lysate immunoprecipitated using ab114968 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse plasmacytoma tissue labelling SPF45 with ab114968 at 1/500 (2µg/ml). Detection: DAB.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab114968 has been referenced in 1 publication.
- Han L et al. A 5'-tRNA halve, tiRNA-Gly promotes cell proliferation and migration via binding to RBM17 and inducing alternative splicing in papillary thyroid cancer. J Exp Clin Cancer Res 40:222 (2021). PubMed: 34225773