Key features and details
- Rabbit polyclonal to SPF45
- Suitable for: IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPF45 antibody
See all SPF45 primary antibodies
DescriptionRabbit polyclonal to SPF45
Tested applicationsSuitable for: IP, IHC-Pmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Orangutan
Synthetic peptide corresponding to Human SPF45 aa 25-75.
Database link: NP_116294.1
- HeLa whole cell lysate.
This product was previously labelled as RBM17
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab114968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionSplice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
Sequence similaritiesContains 1 G-patch domain.
Contains 1 RRM (RNA recognition motif) domain.
- Information by UniProt
- 45 kDa-splicing factor antibody
- RBM 17 antibody
- Rbm17 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling SPF45 with ab114968 at 1/1000 (1µg/ml). Detection: DAB.
Detection of SPF45 by Western Blot of Immunprecipitate.
anti-SPF45 at 0.4µg/ml staining SPF45 in HeLa whole cell lysate immunoprecipitated using ab114968 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab114968 has not yet been referenced specifically in any publications.