Overview

  • Product name

  • Description

    Rabbit polyclonal to SPF45
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SPF45 aa 25-75.
    Database link: NP_116294.1

  • Positive control

    • HeLa whole cell lysate.
  • General notes

     This product was previously labelled as RBM17

     

Properties

Applications

Our Abpromise guarantee covers the use of ab114968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Splice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
    • Sequence similarities

      Contains 1 G-patch domain.
      Contains 1 RRM (RNA recognition motif) domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 45 kDa-splicing factor antibody
      • RBM 17 antibody
      • Rbm17 antibody
      • RNA binding motif protein 17 antibody
      • RNA-binding motif protein 17 antibody
      • SPF 45 antibody
      • SPF45 antibody
      • SPF45_HUMAN antibody
      • Splicing factor (45 kD) antibody
      • Splicing factor (45kD) antibody
      • Splicing factor 45 antibody
      • Splicing factor 45 kDa antibody
      • Splicing factor 45kDa antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling SPF45 with ab114968 at 1/1000 (1µg/ml). Detection: DAB.

    • Detection of SPF45 by Western Blot of Immunprecipitate.
      anti-SPF45 at 0.4µg/ml staining SPF45 in HeLa whole cell lysate immunoprecipitated using ab114968 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 10 seconds.

    References

    ab114968 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab114968.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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