Key features and details
- Rabbit polyclonal to SPF45
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPF45 antibody
See all SPF45 primary antibodies
DescriptionRabbit polyclonal to SPF45
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- RT4 and U251 MG cell lysates; Human tonsil and liver tissue lysates; U2 OS cells; Human lateral ventricle tissue.
This product was previously labelled as RBM17
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab204333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 45 kDa.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
FunctionSplice factor that binds to the single stranded 3'AG at the exon/intron border and promotes its utilization in the second catalytic step. Involved in the regulation of alternative splicing and the utilization of cryptic splice sites. Promotes the utilization of a cryptic splice site created by the beta-110 mutation in the HBB gene. The resulting frameshift leads to sickle cell anemia.
Sequence similaritiesContains 1 G-patch domain.
Contains 1 RRM (RNA recognition motif) domain.
- Information by UniProt
- 45 kDa-splicing factor antibody
- RBM 17 antibody
- Rbm17 antibody
All lanes : Anti-SPF45 antibody (ab204333) at 1/100 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma tissue lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 45 kDa
Immunofluorescent analysis of U2 OS cells (PFA-fixed/Triton X-100 permeabilized) labeling SPF45 with ab204333 at 4 μg/ml (green).
Immunohistochemical analysis of Paraffin-embedded Human lateral ventricle tissue labeling SPF45 with ab204333 at 1/200 dilution.
ab204333 has not yet been referenced specifically in any publications.