Recombinant Anti-Erlin-2 antibody [EPR8088] (ab129207)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8088] to Erlin-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-Erlin-2 antibody [EPR8088]
See all Erlin-2 primary antibodies -
Description
Rabbit monoclonal [EPR8088] to Erlin-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human Erlin-2 aa 50-150. The exact sequence is proprietary.
-
Positive control
- Fetal kidney, 293T, HepG2, and HeLa lysates; Human endometrium adenocarcinoma; HepG2 cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.40 x 10 -13 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8088 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129207 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/100 - 1/250.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
-
Function
Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the band 7/mec-2 family. -
Cellular localization
Endoplasmic reticulum membrane. Associated with lipid raft-like domains of the endoplasmic reticulum membrane. - Information by UniProt
-
Database links
- Entrez Gene: 11160 Human
- Entrez Gene: 244373 Mouse
- Omim: 611605 Human
- SwissProt: O94905 Human
- SwissProt: Q8BFZ9 Mouse
- Unigene: 705490 Human
- Unigene: 277699 Mouse
-
Alternative names
- C8orf2 antibody
- Chromosome 8 open reading frame 2 antibody
- Endoplasmic reticulum lipid raft associated protein 2 antibody
see all
Images
-
All lanes : Anti-Erlin-2 antibody [EPR8088] (ab129207) at 1/1000 dilution
Lane 1 : Fetal kidney tissue lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 38 kDa -
ab129207, at 1/50 dilution, staining Erlin-2 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab129207, at 1/100 dilution, staining Erlin-2 in HepG2 cells by Immunofluorescence.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab129207 has been referenced in 3 publications.
- Agrawal RR et al. Alzheimer's-Associated Upregulation of Mitochondria-Associated ER Membranes After Traumatic Brain Injury. Cell Mol Neurobiol N/A:N/A (2022). PubMed: 36571634
- Rossi AM et al. Quantal Ca2+ release mediated by very few IP3 receptors that rapidly inactivate allows graded responses to IP3. Cell Rep 37:109932 (2021). PubMed: 34731613
- Liu Y et al. Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression. Front Immunol 11:568440 (2020). PubMed: 33424830