Anti-SPG3A/ATL1 antibody [1F6B12] (ab233646)
Key features and details
- Mouse monoclonal [1F6B12] to SPG3A/ATL1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-SPG3A/ATL1 antibody [1F6B12]
See all SPG3A/ATL1 primary antibodies -
Description
Mouse monoclonal [1F6B12] to SPG3A/ATL1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Cynomolgus monkey, Orangutan -
Immunogen
Recombinant fragment corresponding to Human SPG3A/ATL1 aa 1-100. Expressed in E.coli.
Database link: Q8WXF7 -
Positive control
- WB: Recombinant human SPG3A/ATL1 (aa 1-100) protein; SPG3A/ATL1 (aa 1-100)-hIgGFc transfected HEK-293 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1F6B12 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
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Notes |
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WB
1/500 - 1/2000. |
Target
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Function
GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. May also regulate Golgi biogenesis. May regulate axonal development. -
Tissue specificity
Expressed predominantly in the adult and fetal central nervous system. Measurable expression in all tissues examined, although expression in adult brain is at least 50-fold higher than in other tissues. Detected predominantly in pyramidal neurons in the cerebral cortex and the hippocampus of the brain. Expressed in upper and lower motor neurons (at protein level). -
Involvement in disease
Defects in ATL1 are the cause of spastic paraplegia autosomal dominant type 3 (SPG3) [MIM:182600]; also known as Strumpell-Lorrain syndrome. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. -
Sequence similarities
Belongs to the GBP family. Atlastin subfamily. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell projection > axon. - Information by UniProt
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Database links
- Entrez Gene: 102139537 Cynomolgus monkey
- Entrez Gene: 51062 Human
- Entrez Gene: 73991 Mouse
- Entrez Gene: 100173943 Orangutan
- Entrez Gene: 362750 Rat
- Omim: 606439 Human
- SwissProt: Q60HD2 Cynomolgus monkey
- SwissProt: Q8WXF7 Human
see all -
Alternative names
- AD FSP antibody
- atl1 antibody
- ATLA1_HUMAN antibody
see all
Images
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All lanes : Anti-SPG3A/ATL1 antibody [1F6B12] (ab233646) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : SPG3A/ATL1 (aa 1-100)-hlgGFc transfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate -
Anti-SPG3A/ATL1 antibody [1F6B12] (ab233646) at 1/500 dilution + Recombinant human SPG3A/ATL1 protein.
Expected MW is 41kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab233646 has not yet been referenced specifically in any publications.