Overview

  • Product name

  • Description

    Rabbit polyclonal to SPG3A/ATL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human SPG3A/ATL1 (internal sequence). The exact sequence is proprietary.
    Database link: Q8WXF7

  • Positive control

    • WB: Mouse brain tissue extract. ICC/IF: SK-N-SH cells.
  • General notes

     This product was previously labelled as SPG3A

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 64 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. May also regulate Golgi biogenesis. May regulate axonal development.
  • Tissue specificity

    Expressed predominantly in the adult and fetal central nervous system. Measurable expression in all tissues examined, although expression in adult brain is at least 50-fold higher than in other tissues. Detected predominantly in pyramidal neurons in the cerebral cortex and the hippocampus of the brain. Expressed in upper and lower motor neurons (at protein level).
  • Involvement in disease

    Defects in ATL1 are the cause of spastic paraplegia autosomal dominant type 3 (SPG3) [MIM:182600]; also known as Strumpell-Lorrain syndrome. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
  • Sequence similarities

    Belongs to the GBP family. Atlastin subfamily.
  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell projection > axon.
  • Information by UniProt
  • Database links

  • Alternative names

    • AD FSP antibody
    • atl1 antibody
    • ATLA1_HUMAN antibody
    • Atlastin GTPase 1 antibody
    • Atlastin-1 antibody
    • Atlastin1 antibody
    • Brain specific GTP binding protein antibody
    • Brain-specific GTP-binding protein antibody
    • FSP1 antibody
    • GBP-3 antibody
    • GBP3 antibody
    • GTP-binding protein 3 antibody
    • Guanine nucleotide-binding protein 3 antibody
    • Guanylate binding protein 3 antibody
    • hGBP3 antibody
    • HSN1D antibody
    • Spastic paraplegia 3 protein A antibody
    • SPG 3A antibody
    • SPG3 antibody
    • SPG3A antibody
    • SPG3A gene antibody
    see all

Images

  • SK-N-SH (Human neuroblastoma cell line) cells stained for SPG3A/ATL1 (green) using ab229582 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).

  • Anti-SPG3A/ATL1 antibody (ab229582) at 1/1000 dilution + Mouse brain tissue extract at 50 µg

    Predicted band size: 64 kDa



    7.5% SDS-PAGE gel.

References

ab229582 has not yet been referenced specifically in any publications.

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