Anti-SPG3A/ATL1 antibody (ab229582)
Key features and details
- Rabbit polyclonal to SPG3A/ATL1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SPG3A/ATL1 antibody
See all SPG3A/ATL1 primary antibodies -
Description
Rabbit polyclonal to SPG3A/ATL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Rhesus monkey
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Immunogen
Recombinant fragment within Human SPG3A/ATL1 (internal sequence). The exact sequence is proprietary.
Database link: Q8WXF7 -
Positive control
- WB: Mouse brain tissue extract. ICC/IF: SK-N-SH cells.
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General notes
This product was previously labelled as SPG3A
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab229582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 64 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. May also regulate Golgi biogenesis. May regulate axonal development. -
Tissue specificity
Expressed predominantly in the adult and fetal central nervous system. Measurable expression in all tissues examined, although expression in adult brain is at least 50-fold higher than in other tissues. Detected predominantly in pyramidal neurons in the cerebral cortex and the hippocampus of the brain. Expressed in upper and lower motor neurons (at protein level). -
Involvement in disease
Defects in ATL1 are the cause of spastic paraplegia autosomal dominant type 3 (SPG3) [MIM:182600]; also known as Strumpell-Lorrain syndrome. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. -
Sequence similarities
Belongs to the GBP family. Atlastin subfamily. -
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell projection > axon. - Information by UniProt
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Database links
- Entrez Gene: 51062 Human
- Entrez Gene: 73991 Mouse
- Entrez Gene: 362750 Rat
- Omim: 606439 Human
- SwissProt: Q8WXF7 Human
- SwissProt: Q8BH66 Mouse
- SwissProt: Q6PST4 Rat
- Unigene: 584905 Human
see all -
Alternative names
- AD FSP antibody
- atl1 antibody
- ATLA1_HUMAN antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SPG3A/ATL1 antibody (ab229582)SK-N-SH (Human neuroblastoma cell line) cells stained for SPG3A/ATL1 (green) using ab229582 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
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Western blot - Anti-SPG3A/ATL1 antibody (ab229582)Anti-SPG3A/ATL1 antibody (ab229582) at 1/1000 dilution + Mouse brain tissue extract at 50 µg
Predicted band size: 64 kDa7.5% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab229582 has not yet been referenced specifically in any publications.
