Key features and details
- Rabbit polyclonal to SPG48
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPG48 antibody
DescriptionRabbit polyclonal to SPG48
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- WB: HL-60 cell lysate. IHC-P: Human stomach tissue. ICC/IF: U-2 OS cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 49,67,89 kDa.|
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
RelevanceThe function of SPG48 remains unknown. There are 3 named isoforms produced by alternative splicing.
- Hypothetical protein LOC9907 antibody
- KIAA0415 antibody
- Uncharacterized protein KIAA0415 antibody
Paraffin-embedded human stomach tissue stained for SPG48 using ab224153 at 1/200 dilution in immunohistochemical analysis.
Anti-SPG48 antibody (ab224153) at 1/250 dilution + HL-60 (human promyelocytic leukemia cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 49,67,89 kDa
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for SPG48 (green) using ab224153 at 4 µg/ml in ICC/IF.
ab224153 has not yet been referenced specifically in any publications.