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Spastic paraplegia 7 (pure and complicated autosomal recessive) antibody
Spastic paraplegia 7 homolog (human) antibody
Spastic paraplegia 7 protein antibody
Spastic paraplegia 7, paraplegin (pure and complicated autosomal recessive) antibody
Spastic paraplegia protein 7 antibody
SPG7 gene antibody
Western blot - Anti-SPG7 antibody (ab154989)
All lanes : Anti-SPG7 antibody (ab154989) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889) Lane 2 : Human spinal cord tissue lysate - total protein (ab29188) Lane 3 : Spinal Cord (Mouse) Tissue Lysate Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
The predicted molecular weight of SPG7 is 88 kDa (SwissProt), however we expect to observe a banding pattern around 100 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab154989 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
Douglas JN et al. Antibodies to the RNA Binding Protein Heterogeneous Nuclear Ribonucleoprotein A1 Colocalize to Stress Granules Resulting in Altered RNA and Protein Levels in a Model of Neurodegeneration in Multiple Sclerosis. J Clin Cell Immunol7:402 (2016).
Read more (PubMed: 27375925) »