Overview

  • Product name

    Anti-Sphingomyelin Synthase 1 antibody
    See all Sphingomyelin Synthase 1 primary antibodies
  • Description

    Rabbit polyclonal to Sphingomyelin Synthase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids 154-203 (LTTVMISVVH ERVPPKEVQP PLPDTFFDHF NRVQWAFSIC EINGMILVGL) of mouse Sphingomyelin Synthase 1 (NP_659041).

  • Positive control

    • Mouse muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab135365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.

Target

  • Relevance

    Sphingomyelin synthase 1 is a bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Sphingomyelin synthase 1 is required for cell growth and is also capable of suppressing BAX-mediated apoptosis and preventing cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. It may protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.
  • Cellular localization

    Golgi Apparatus. Multi-pass membrane protein
  • Database links

  • Alternative names

    • MOB antibody
    • Phosphatidylcholine:ceramide cholinephosphotransferase 1 antibody
    • Protein Mob antibody
    • SGMS1 antibody
    • SMS 1 antibody
    • SMS1 antibody
    • TMEM23 antibody
    • Transmembrane protein 23 antibody
    see all

Images

  • Anti-Sphingomyelin Synthase 1 antibody (ab135365) at 1 µg/ml + Mouse muscle tissue lysate at 10 µg

    Predicted band size: 49 kDa

References

ab135365 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophage)
Gel Running Conditions
Reduced Denaturing (gel 10%)
Loading amount
20 µg
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

美弘 吉川

Verified customer

Submitted Sep 28 2016

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