Key features and details
- Rabbit polyclonal to Sphingomyelin Synthase 1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Sphingomyelin Synthase 1 antibody
See all Sphingomyelin Synthase 1 primary antibodies
DescriptionRabbit polyclonal to Sphingomyelin Synthase 1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig
- WB: HeLa and HepG2 whole cell lysate (ab7900). IHC-P: Human pancreatic tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab235057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Predicted molecular weight: 49 kDa.|
|IHC-P||1/200 - 1/500.|
RelevanceSphingomyelin synthase 1 is a bidirectional lipid cholinephosphotransferase capable of converting phosphatidylcholine and ceramide to sphingomyelin (SM) and diacylglycerol (DAG) and vice versa. Sphingomyelin synthase 1 is required for cell growth and is also capable of suppressing BAX-mediated apoptosis and preventing cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. It may protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.
Cellular localizationGolgi Apparatus. Multi-pass membrane protein
- MOB antibody
- Phosphatidylcholine:ceramide cholinephosphotransferase 1 antibody
- Protein Mob antibody
All lanes : Anti-Sphingomyelin Synthase 1 antibody (ab235057) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Paraffin-embedded human pancreatic tissue stained for Sphingomyelin Synthase 1 using ab235057 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. Primary antibody (1% BSA) was incubated at 4°C overnight. The primary antibody was detected by a biotinylated secondary antibody and visualized using a HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235057 has not yet been referenced specifically in any publications.