Key features and details
- Rabbit polyclonal to SPHK1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPHK1 antibody
See all SPHK1 primary antibodies
DescriptionRabbit polyclonal to SPHK1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human SPHK1 (internal sequence). The exact sequence is proprietary.
Database link: Q9NYA1
- WB: MOLT-4 whole cell lysate. ICC/IF: A-375 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 43 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
Tissue specificityWidely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
Sequence similaritiesContains 1 DAGKc domain.
- Information by UniProt
- SK 1 antibody
- SK1 antibody
- Sphingosine kinase 1 antibody
Anti-SPHK1 antibody (ab228645) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS-PAGE gel.
4% paraformaldehyde-fixed A-375 (human malignant melanoma cell line) cells stained for SPHK1 (green) using ab228645 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228645 has not yet been referenced specifically in any publications.