Overview

  • Product name

  • Description

    Rabbit polyclonal to SPHK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human SPHK1 (internal sequence). The exact sequence is proprietary.
    Database link: Q9NYA1

  • Positive control

    • WB: MOLT-4 whole cell lysate. ICC/IF: A-375 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
  • Tissue specificity

    Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
  • Sequence similarities

    Contains 1 DAGKc domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • SK 1 antibody
    • SK1 antibody
    • Sphingosine kinase 1 antibody
    • SPHK 1 antibody
    • SPHK antibody
    • Sphk1 antibody
    • SPHK1_HUMAN antibody
    • SPK antibody
    • SPK 1 antibody
    see all

Images

  • Anti-SPHK1 antibody (ab228645) at 1/1000 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate at 30 µg

    Predicted band size: 43 kDa



    10% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed A-375 (human malignant melanoma cell line) cells stained for SPHK1 (green) using ab228645 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab228645 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228645.
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