Overview

  • Product name

  • Description

    Rabbit polyclonal to SPHK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Non human primates
  • Immunogen

    Synthetic peptide corresponding to Human SPHK1 aa 286-315 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 8877
    (Peptide available as ab187382)

  • Positive control

    • WB: 293, HepG2, HUVEC, Raji, C6 and C2C12 cell lysates. Mouse kidney tissue lysate. IHC-P: Human hepatocarcinoma tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab71700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 43 kDa).

There are two known isoforms of this protein, one predicted around 43 kDa and the other around 51 kDa.

Target

Images

  • All lanes : Anti-SPHK1 antibody (ab71700) at 1/1000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HUVEC cell lysate
    Lane 4 : Raji cell lysate
    Lane 5 : C6 cell lysate
    Lane 6 : C2C12 cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 43 kDa

  • Immunohistochemical staining of SPHK1 in Human hepatocarcinoma tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab71700 at a dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A HRP-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • ICC/IF image of ab71700 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71700, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-SPHK1 antibody (ab71700) at 1/500 dilution + Lysate prepared from human MDA MB 231 cells at 15 µg

    Secondary
    HRP-conjugated donkey polyclonal to rabbit IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 48,52 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 minutes

    See Abreview

  • Anti-SPHK1 antibody (ab71700) at 1/1000 dilution + mouse kidney tissue lysate at 12.5 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/5000 dilution

    Predicted band size: 43 kDa
    Observed band size: 48 kDa why is the actual band size different from the predicted?
    Additional bands at: 52 kDa. We are unsure as to the identity of these extra bands.



    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:

  • Cao XZ  et al. MiR-128 suppresses the growth of thyroid carcinoma by negatively regulating SPHK1. Biomed Pharmacother 109:1960-1966 (2019). Read more (PubMed: 30551451) »
  • Speirs MMP  et al. Imbalanced sphingolipid signaling is maintained as a core proponent of a cancerous phenotype in spite of metabolic pressure and epigenetic drift. Oncotarget 10:449-479 (2019). Read more (PubMed: 30728898) »
See all 18 Publications for this product

Customer reviews and Q&As

1-10 of 14 Abreviews or Q&A

Question
Answer

We have previously been in contact regarding ab71700, which was not providing satisfactory results.

A free of charge replacement vial was issued and I am writing to inquire if the replacement product has worked. I would appreciate if you could let me know whether satisfactory results were obtained with this new vial as I am keen to know how you are progressing.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, please do not hesitate to let us know.

I wish you the best of luck with your research and please do not hesitate to contact us if you have any further questions.

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Question
Answer

Thank you for contacting us and reporting the problems observed with lot inconsistency with Anti-SPHK1 antibody (ab71700).


I am currently looking into whether there has been any lot inconsistency observed with this antibody and if we potentially have any of the original lot you received. If you could send me the data which you have collected with the new batch that would be very helpful in allowing me to more fully understand the problems you have been experiencing.


I am sorry for any inconvenience this has caused.

Read More

Answer

Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Le numéro de commande de remplacement gratuit avec la référence ab37977est : *****.

Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

N'hésitez pas à nous contacter lors d'une prochaine occasion.

Read More

Answer

Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés.
Comme nous avons discuté au téléphone, ces informations sont très importantes pour nous. Ils nous permettent d'abord de cibler le problème et déterminer l'origine du problème ainsi que d’initier des investigations internes si nécessaire. Souvent des modifications du protocole peuvent résoudre la problème. Si nous concluons que le produit que vous avez reçu ne fonctionne pas comme mentionné sur sa fiche technique et que ce produit a été commandé sous 180 jours, nous nous ferons un plaisir de vous offrir un remplacement gratuit ou un avoir en compensation.

J'apprécierais donc si vous pouviez confirmer ou répondre au points suivants, qui ne sont pas claire après réception du questionnaire:

1.) Est-ce que vous avez déjà utilisé des autres lots de cet anticorps, qui vous donnaient des bons résultats? Si oui, lesquels et à quel dilution?

2.) Quel tissue est-ce que vous avez utilisé? Est-ce que le tissue est connue pour l'expression de SPHK1?

3.) Est-ce que vous avez fait des marquages avec des autres anticorps sur ces même blocs de tissu afin de s'assurer de la bonne qualité du bloc?

4.) Est-ce que vous avez déjà utilisé les réactifs de détection avec un autre anticorps primaire de lapin avec des bons résultats?

5.) Quel tampon de blocage est-ce que vous utilisez? Est-ce que vous pourriez envisager un autre tampon de blocage?

Finalement, pouvez vous aussi me confirmer le numéro de commande ou la date de commande avec le nom de la personne qui passait la commande?

Merci beaucoup de votre coopération. Je meréjouis de votre réponse afin de pouvoirmieux comprendre le problème.

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Question
Answer

Merci pour votre appel ce matin. Je suis désolée d'apprendre aussi que ab71700 ne vous donne pas de bons résultats en immunohistochimie.

Veuillez trouver ci-joint lequestionnaire discuté au téléphone qui nous permet de regrouper le plus d'informations possible sur le protocole que vous avez utilisé avec cet anticorps.
Celui-ci nous permettra de vous fournir la meilleure assistance technique.

Si nous concluons que notre produit est fautif et ne fonctionne pas comme explicité par notre fiche technique, nous serons ravis de mettre en place l'envoi d'un tube de remplacement gratuit ou d'un remboursement (sous réserve d'une commande ultérieure de 180 jours).

Je vous remercie par avance de nous envoyer votre protocole.

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Question
Answer

According to our records, ab71700 was proving difficult to use inWB and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced. If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.

If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.

We wish you the best of luck with your research and look forward to a reply.

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Answer


I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.
Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.
The credit note ID is for your reference only and does not automatically guarantee the credit.
I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Answer

Thanks for your email. Sorry to hear this vial of ab71700 is giving you trouble.
If you have purchased this antibody within the last 6 months or so I would be happy to provide a free replacement antibody. In your reply, please include your PO number or Abcam order reference number, as well as the catalog number of the replacement antibody you are interested in, and I will be happy to arrange things.
I look forward to hearing from you.

Read More

Question

Dear Technical Team,

Thank you for your mail.

Please find below the reply from the customer & also arrange for the needful to update the customer accordingly on it.

Looking forward towards your valued response on it.

With Best Regards,


Dear Sir,

Yes, the same secondary antibody is being used for all these antibodies, even being used with primary antibodyof other brands, working well.

Please replace these antibodies.

regards,














----- Original Message -----

From: mailto:technical@abcam.com

To: mailto:nulife@bol.net.in

Sent: Wednesday, January 25, 2012 3:56 PM

Subject: Reply from Abcam to your enquiry regarding ab14672, ab60532, ab71700, ab81505 [CCE3453323]



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https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header









Dear Nadeem



We have an answer to your inquiry:




Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.



The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. I would like to reassure you that these antibodiesare tested and covered by our 6 month guarantee forWB. All the antibodies, except for ab81505Arg2 antibody,are guaranteed for rat samples.



Reviewing this case, I would like to offer some suggestions to help optimize the results. I would also appreciate if you can confirm some further details:



1. Please confirm the Abcam order reference number and date of purchase for each of these antibodies.



2. Could you confirm the same secondary antibody is being used for allof these antibodies? Is the current vial of secondary antibody working well with any other primary antibodies? Please provide further details of the secondary (supplier and catalog number).



3. I can recommend to try somefresh samples.



4. I can suggest to include a loading control such as GAPDH in order to assess the quality of the sample and the transfer to the membrane.



5. I can recommend to include some positive controls if they have not already been tried. Reviewing the information on the datasheets, anddetails fromthe SwissProt/Uniprot protein database links, I can suggest for example:



PDE5A (ab 14672)

Rat lung homogenate should already be a suitable control



cGK1 (ab 60532)

Epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus express cGK1,



Sphk1 (ab 71700)

I am sorry I am not able to confirm what expression level isexpected in lung? Do you have any further information? I can suggest adult liver, kidney, heartor skeletal muscle would be a suitable control.



4. Arg2 (ab 81505)

I am sorry I am not able to confirm whatexpression level is expected in the lung? Do you have any further information? This protein is expressed most strongly in kidney and prostate,

I can recommend to try a humansample positive control as human samlples are tested and covered by the guarantee. Rat samples arepredicted only due to high sequence alignment, I am sorry they have not been tested.



In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.



I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.





Help us improve our service.
https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=3453323




Your original inquiry to Abcam:





Dear Technical Team,







Hope this e-mail finds you well!








Kindly look into the belowmail from one this customer & advise us on it, enabling us to update him accordingly.







Looking forward towards your valued response on it.







With Best Regards,










Dear Sir,







We procured the following antibodies from you via supply order : 201/DIPAS

1. PDE5A (ab 14672)

2. cGK1 (ab 60532)

3. Sphk1 (ab 71700)

4. Arg2 (ab 81505)



we have been testing these antibodies for the past 3 months and their functionality is now being doubted as we have loaded increasing

amounts of protein sample during western blot experiments (starting with 30ug - till 50 ug) and tried extended primary incubations

(3hr/5hr/overnight) at the recommended antibody concentrations (1:1000-1:500), but there seems to be no binding of the primary

antibody to the blots as observed on developing through ECL detection system (SIGMA).This hasseriouslyaffectedprogressof our research project.





kindly take notice of the problem and if possible try to replace the above, as the above purchases are still in the warranty period.







Enclosed please find the required information for further action.





regards,


















Order Details

Antibody code :

Problem :

- No Signal or Weak Signal

Lot number :

Purchase order number : 201/DIPAS/MMG/DIP -251/111/11-12/A2.3

or preferably Abcam order number





General Information

Antibody storage conditions (temperature/reconstitution etc)

Stored at -20°C in aliquots and working dilutions stored at 4°C.





Description of the problem (high background, wrong band size, more bands, no band etc.)

No bands



Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)

Rat whole lung homogenate prepared in RIPA lysis buffer



Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)

RIPA lysis buffer fortified with 1mM PMSF and protease inhibitor cocktail (Roche). Sample was appropriately diluted with 6X Laemelli buffer and water and boiled for 10 minutes in a water bath.



Amount of protein loaded

Started with 30μg and increased the protein loaded/well till 50 μg

Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)

Reducing gel (SDS-PAGE) , 5% stacking and 10% resolving. Gel was run at 60V for 4 hours after stacking at 55V.

Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)

Transfer : 15V at room temperature for 1hr in a semi-dry blotting apparatus.

Transfer buffer: 0.025M Tris base, 0.192M Glycine, 20% Methanol (pH 8.3)

Blocking Buffer: 5% BSA in TBST

Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

PDE5A (ab14672), Sphk1(ab 71700), Arg 2( ab81505) : all manufactured by Abcam

Species reactivity : Human, Mouse, Rat

Origin: Rabbit

Dilutions: 1:500

Diluent: TBST+ 5% BSA

Incubation time: 3 hours – overnight (at 37°C / 4°C, as suitable)

Washing: 3-5 washings for 10 minutes with TBST at room temperature

Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

Anti-Rabbit IgG (Santa Cruz)

Origin: Goat

Dilution: 1:5000

Diluent: TBST+5%BSA

Incubation time: 2-3 hours at 37°C

Washing: 3-5 washings for 10 minutes with TBST at room temperature

Detection method (ECL, ECLPlus etc.)

Detection Method: ECL (SIGMA) + Kodak Blue Sensitive X-Ray Film+ Kodak Dental developer/fixer



Positive and negative controls used (please specify)

Positive control: Blots probed with above specifies antibodies were stripped, blocked and re-probed for β-actin. Specific bands of β-actin were obtained on the exposed film.



Optimization attempts (problem solving)

How many times have you tried the Western?



9 times for each antibody



Have you run a "No Primary" control? Yes No Not required



Do you obtain the same results every time? Yes No

e.g. are the background bands always in the same place?

No, only blank x-ray film was obtained each time





What steps have you altered?

- We have increased the protein concentration loaded/well from 30ug to 50ug.

- Used the recommended antibody dilutions at high concentration and longer incubation periods – 3 hours to overnight.

- The transfer efficiency was ensured by ponceau staining.

Additional Notes

Attached images are representative of each antibody probing procedure (only the highest concentration of protein loaded with longest primary antibody incubation has been depicted in the images)












Best regards,
Kate

Kate Hayes
Scientific Support Supervisor
Abcam plc
www.abcam.com



Abcam Customer Services and Scientific Support Team
https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

[CCE3453323]










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Read More
Answer

Thank you for providing this further information.

I appreciate your cooperation and understand your concerns. It is regrettable the results have not been succesful.

In order to help with our invesigation of this case before deciding how to proceed, I would appreciate if you are able to provide more detail as requested in the previous email. This information will also be helpful to our quality monitoring.

1. Please confirm the Abcam order reference number and date of purchase for each of these antibodies.

2. I can suggest to include a loading control such as GAPDH in order to assess the quality of the sample and the transfer to the membrane.
Could you confirm the results from the loading control?

3. I can recommend to include some positive controls if they have not already been tried.

Please confirm what level of expression is expected in the samples. Which positive controls have been tried for each antibody?

Particularly for ab81505 which has not been tested and guaranteed in rat, please confirm if a human positive control has been tried as suggested.

Thank you for your time. I look forward to hearing from you with the requested information and hope we can resolve this case as soon as possible.

Read More

Question

Dear Technical Team,

Hope this e-mail finds you well!

Kindly look into the belowmail from one this customer & advise us on it, enabling us to update him accordingly.

Looking forward towards your valued response on it.

With Best Regards,


Dear Sir,

We procured the following antibodies from you via supply order : 201/DIPAS
1. PDE5A (ab 14672)
2. cGK1 (ab 60532)
3. Sphk1 (ab 71700)
4. Arg2 (ab 81505)

we have been testing these antibodies for the past 3 months and their functionality is now being doubted as we have loaded increasing
amounts of protein sample during western blot experiments (starting with 30ug - till 50 ug) and tried extended primary incubations
(3hr/5hr/overnight) at the recommended antibody concentrations (1:1000-1:500), but there seems to be no binding of the primary
antibody to the blots as observed on developing through ECL detection system (SIGMA).This hasseriouslyaffectedprogressof our research project.

kindly take notice of the problem and if possible try to replace the above, as the above purchases are still in the warranty period.

Enclosed please find the required information for further action.

regards,






Order Details
Antibody code :
Problem :
- No Signal or Weak Signal
Lot number :
Purchase order number : 201/DIPAS/MMG/DIP -251/111/11-12/A2.3
or preferably Abcam order number

General Information
Antibody storage conditions (temperature/reconstitution etc)
Stored at -20°C in aliquots and working dilutions stored at 4°C.

Description of the problem (high background, wrong band size, more bands, no band etc.)
No bands
Sample (Species/Cell extract/Nuclear extract/Purified protein/Recombinant protein etc.)
Rat whole lung homogenate prepared in RIPA lysis buffer
Sample preparation (Buffer/Protease inhibitors/Heating sample etc.)
RIPA lysis buffer fortified with 1mM PMSF and protease inhibitor cocktail (Roche). Sample was appropriately diluted with 6X Laemelli buffer and water and boiled for 10 minutes in a water bath.
Amount of protein loaded
Started with 30μg and increased the protein loaded/well till 50 μg
Electrophoresis/Gel conditions (Reducing or Non-reducing gel, % of the gel etc.)
Reducing gel (SDS-PAGE) , 5% stacking and 10% resolving. Gel was run at 60V for 4 hours after stacking at 55V.
Transfer and blocking conditions (Buffer/time period, Blocking agent etc.)
Transfer : 15V at room temperature for 1hr in a semi-dry blotting apparatus.
Transfer buffer: 0.025M Tris base, 0.192M Glycine, 20% Methanol (pH 8.3)
Blocking Buffer: 5% BSA in TBST
Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
PDE5A (ab14672), Sphk1(ab 71700), Arg 2( ab81505) : all manufactured by Abcam
Species reactivity : Human, Mouse, Rat
Origin: Rabbit
Dilutions: 1:500
Diluent: TBST+ 5% BSA
Incubation time: 3 hours – overnight (at 37°C / 4°C, as suitable)
Washing: 3-5 washings for 10 minutes with TBST at room temperature
Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)
Anti-Rabbit IgG (Santa Cruz)
Origin: Goat
Dilution: 1:5000
Diluent: TBST+5%BSA
Incubation time: 2-3 hours at 37°C
Washing: 3-5 washings for 10 minutes with TBST at room temperature
Detection method (ECL, ECLPlus etc.)
Detection Method: ECL (SIGMA) + Kodak Blue Sensitive X-Ray Film+ Kodak Dental developer/fixer
Positive and negative controls used (please specify)
Positive control: Blots probed with above specifies antibodies were stripped, blocked and re-probed for β-actin. Specific bands of β-actin were obtained on the exposed film.
Optimization attempts (problem solving)
How many times have you tried the Western?
9 times for each antibody
Have you run a "No Primary" control? Yes No Not required
Do you obtain the same results every time? Yes No
e.g. are the background bands always in the same place?
No, only blank x-ray film was obtained each time

What steps have you altered?
- We have increased the protein concentration loaded/well from 30ug to 50ug.
- Used the recommended antibody dilutions at high concentration and longer incubation periods – 3 hours to overnight.
- The transfer efficiency was ensured by ponceau staining.
Additional Notes
Attached images are representative of each antibody probing procedure (only the highest concentration of protein loaded with longest primary antibody incubation has been depicted in the images)

Read More
Answer

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality. I would like to reassure you that these antibodiesare tested and covered by our 6 month guarantee forWB. All the antibodies, except for ab81505Arg2 antibody,are guaranteed for rat samples.

Reviewing this case, I would like to offer some suggestions to help optimize the results. I would also appreciate if you can confirm some further details:

1. Please confirm the Abcam order reference number and date of purchase for each of these antibodies.

2. Could you confirm the same secondary antibody is being used for allof these antibodies? Is the current vial of secondary antibody working well with any other primary antibodies? Please provide further details of the secondary (supplier and catalog number).

3. I can recommend to try somefresh samples.

4. I can suggest to include a loading control such as GAPDH in order to assess the quality of the sample and the transfer to the membrane.

5. I can recommend to include some positive controls if they have not already been tried. Reviewing the information on the datasheets, anddetails fromthe SwissProt/Uniprot protein database links, I can suggest for example:

PDE5A (ab 14672)
Rat lung homogenate should already be a suitable control

cGK1 (ab 60532)
Epithelial cells of thymus, dendritic cells of lymph node, and in the basal cell layer of epithelia such as epidermis, nasopharynx, respiratory tract, salivary gland, and esophagus express cGK1,

Sphk1 (ab 71700)
I am sorry I am not able to confirm what expression level isexpected in lung? Do you have any further information? I can suggest adult liver, kidney, heartor skeletal muscle would be a suitable control.

4. Arg2 (ab 81505)
I am sorry I am not able to confirm whatexpression level is expected in the lung? Do you have any further information? This protein is expressed most strongly in kidney and prostate,

I can recommend to try a humansample positive control as human samlplese are tested and covered by the guarantee. Rat samples arepredicted only due to high sequence alignment, I am sorry they have not been tested.

In the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Read More

1-10 of 14 Abreviews or Q&A

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