Product nameAnti-SPHK1 antibody [EPR4293(2)]
See all SPHK1 primary antibodies
DescriptionRabbit monoclonal [EPR4293(2)] to SPHK1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human SPHK1 aa 50-150. The exact sequence is proprietary.
- Raji, JAR, HUVEC or HT-1080 lysate Permeabilized Raji cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab109522 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 43 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
Tissue specificityWidely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
Sequence similaritiesContains 1 DAGKc domain.
- Information by UniProt
- SK 1 antibody
- SK1 antibody
- Sphingosine kinase 1 antibody
All lanes : Anti-SPHK1 antibody [EPR4293(2)] (ab109522) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : JAR cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : HT-1080 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Flow cytometric analysis of permeabilized Raji cells using ab109522 (red), or a rabbit IgG (negative) (green).
ab109522 has not yet been referenced specifically in any publications.