Overview

  • Product name

    Anti-SPHK1 antibody [EPR4293(2)]
    See all SPHK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4293(2)] to SPHK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SPHK1 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Raji, JAR, HUVEC or HT-1080 lysate Permeabilized Raji cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 43 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-SPHK1 antibody [EPR4293(2)] (ab109522) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : HT-1080 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 43 kDa
      Observed band size: 51 kDa
      why is the actual band size different from the predicted?

    • Flow cytometric analysis of permeabilized Raji cells using ab109522 (red), or a rabbit IgG (negative) (green).

    References

    ab109522 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109522.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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