Product nameSPHK1 overexpression 293T lysate (whole cell)
ab94163 is a 293T cell transfected lysate in which Human SPHK1 has been transiently over-expressed using a pCMV-SPHK1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
BackgroundFunction: Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. Tissue specificity: Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle. Similarity: Contains 1 DAGKc domain.
Our Abpromise guarantee covers the use of ab94163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94163 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-SPHK1 antibody (ab56414) at 1/500 dilution
Lane 1 :
SPHK1 overexpression 293T lysate (whole cell) (ab94163)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94163 has not yet been referenced specifically in any publications.