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Cell Biology Apoptosis Intracellular Kinases
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Anti-SPHK2 antibody (ab264042)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-SPHK2 antibody (ab264042)
  • Western blot - Anti-SPHK2 antibody (ab264042)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPHK2 antibody (ab264042)

Key features and details

  • Rabbit polyclonal to SPHK2
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SPHK2 antibody
    See all SPHK2 primary antibodies
  • Description

    Rabbit polyclonal to SPHK2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human SPHK2 aa 100-200 conjugated to keyhole limpet haemocyanin.
    Database link: Q9NRA0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse stomach lysate. IHC-P: Mouse testis tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Lipid Kinases
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • Mouse stomach normal tissue lysate - total protein (ab29256)
  • Recombinant Protein

    • Recombinant Mouse SPHK2 protein (ab61643)
  • Related Products

    • Recombinant human SPHK2 protein (ab125634)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab264042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Mouse
WB
Mouse
Application Abreviews Notes
IHC-P
1/200 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/300 - 1/1000. Predicted molecular weight: 69 kDa.
ICC/IF
1/50 - 1/200.
Notes
IHC-P
1/200 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/300 - 1/1000. Predicted molecular weight: 69 kDa.
ICC/IF
1/50 - 1/200.

Target

  • Function

    Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
  • Sequence similarities

    Contains 1 DAGKc domain.
  • Cellular localization

    Cytoplasm. Membrane and Lysosome membrane.
  • Target information above from: UniProt accession Q9NRA0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 56848 Human
    • Entrez Gene: 56632 Mouse
    • Omim: 607092 Human
    • SwissProt: Q9NRA0 Human
    • SwissProt: Q9JIA7 Mouse
    • Unigene: 528006 Human
    • Unigene: 24222 Mouse
    • Alternative names

      • SK 2 antibody
      • Sphingosine kinase 2 antibody
      • Sphingosine kinase type 2 antibody
      • SPHK2 antibody
      • SPHK2_HUMAN antibody
      • SPK 2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SPHK2 antibody (ab264042)
      Immunocytochemistry/ Immunofluorescence - Anti-SPHK2 antibody (ab264042)

      4% Paraformaldehyde-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SPHK2 using ab264042 at 1/100 dilution for 90 minutes at 37°C (green). Secondary antibody is an FITC-conjugated Goat anti-rabbit IgG incubated for 90 minutes at 37°C.

      The nuclear counterstain is DAPI (blue).

      Blocked with normal goat serum for 20 minutes at 37°C.

    • Western blot - Anti-SPHK2 antibody (ab264042)
      Western blot - Anti-SPHK2 antibody (ab264042)
      Anti-SPHK2 antibody (ab264042) at 1/300 dilution + Mouse stomach lysate

      Predicted band size: 69 kDa



      Primary incubation overnight at 4°C.

      Secondary incubation for 60 minutes at 37°C.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPHK2 antibody (ab264042)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPHK2 antibody (ab264042)

      Paraformaldehyde-fixed, paraffin-embedded mouse testis tissue stained for SPHK2 using ab264042 at 1/400 overnight at 4°C, followed by an FITC-conjugated Goat anti-rabbit IgG for 90 minutes (green). The nuclear counterstain is DAPI (blue).

      Antigen retrieval: Sodium citrate buffer (pH 6.0) for 15 minutes.

      Blocking: Normal goat serum for 30 minutes at 37°C.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab264042? Please let us know so that we can cite the reference in this datasheet.

    ab264042 has not yet been referenced specifically in any publications.

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