Anti-SPHK2 antibody (ab264042)
Key features and details
- Rabbit polyclonal to SPHK2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-SPHK2 antibody
See all SPHK2 primary antibodies -
Description
Rabbit polyclonal to SPHK2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P MouseWB Mouse
-
Immunogen
Synthetic peptide corresponding to Human SPHK2 aa 100-200 conjugated to keyhole limpet haemocyanin.
Database link: Q9NRA0 -
Positive control
- WB: Mouse stomach lysate. IHC-P: Mouse testis tissue. ICC/IF: HeLa cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| IHC-P |
Mouse
|
| WB |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/200 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
1/300 - 1/1000. Predicted molecular weight: 69 kDa.
|
|
| ICC/IF |
1/50 - 1/200.
|
| Notes |
|---|
|
IHC-P
1/200 - 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/300 - 1/1000. Predicted molecular weight: 69 kDa. |
|
ICC/IF
1/50 - 1/200. |
Target
-
Function
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides. -
Sequence similarities
Contains 1 DAGKc domain. -
Cellular localization
Cytoplasm. Membrane and Lysosome membrane. - Information by UniProt
-
Database links
- Entrez Gene: 56848 Human
- Entrez Gene: 56632 Mouse
- Omim: 607092 Human
- SwissProt: Q9NRA0 Human
- SwissProt: Q9JIA7 Mouse
- Unigene: 528006 Human
- Unigene: 24222 Mouse
-
Alternative names
- SK 2 antibody
- Sphingosine kinase 2 antibody
- Sphingosine kinase type 2 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-SPHK2 antibody (ab264042)
4% Paraformaldehyde-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SPHK2 using ab264042 at 1/100 dilution for 90 minutes at 37°C (green). Secondary antibody is an FITC-conjugated Goat anti-rabbit IgG incubated for 90 minutes at 37°C.
The nuclear counterstain is DAPI (blue).
Blocked with normal goat serum for 20 minutes at 37°C.
-
Western blot - Anti-SPHK2 antibody (ab264042)Anti-SPHK2 antibody (ab264042) at 1/300 dilution + Mouse stomach lysate
Predicted band size: 69 kDaPrimary incubation overnight at 4°C.
Secondary incubation for 60 minutes at 37°C.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPHK2 antibody (ab264042)Paraformaldehyde-fixed, paraffin-embedded mouse testis tissue stained for SPHK2 using ab264042 at 1/400 overnight at 4°C, followed by an FITC-conjugated Goat anti-rabbit IgG for 90 minutes (green). The nuclear counterstain is DAPI (blue).
Antigen retrieval: Sodium citrate buffer (pH 6.0) for 15 minutes.
Blocking: Normal goat serum for 30 minutes at 37°C.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab264042 has not yet been referenced specifically in any publications.