Product nameAnti-SPHK2 (phospho T578) antibody
See all SPHK2 primary antibodies
DescriptionRabbit polyclonal to SPHK2 (phospho T578)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human SPHK2 (phospho T578) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9NRA0
- HeLa cell lysate treated with calyculin A or lambda phosphatase; Recombinant Human SPHK2 protein.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab215750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 69 kDa.|
FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
Sequence similaritiesContains 1 DAGKc domain.
Cellular localizationCytoplasm. Membrane and Lysosome membrane.
- Information by UniProt
- SK 2 antibody
- Sphingosine kinase 2 antibody
- Sphingosine kinase type 2 antibody
Lanes 1-2 & 5-6 : anti-SPHK2 (N-terminal)
Lanes 3-4 & 7-8 : Anti-SPHK2 (phospho T578) antibody (ab215750) at 1/1000 dilution
Lanes 1 & 3 : recombinant Human SPHK2 protein
Lane 2 : recombinant Human SPHK2 protein treated with lambda phosphatase
Lane 4 : recombinant Human SPHK2 protein lambda phosphatase treated
Lane 5 : HeLa cell lysate calyculin A treated
Lanes 6 & 8 : HeLa cell lysate calyculin A and lambda phosphatase treated
Lane 7 : HeLa cell lysate calyculin A treated
Predicted band size: 69 kDa
ab215750 has not yet been referenced specifically in any publications.