Key features and details
- Rabbit polyclonal to SPHK2 (phospho T578)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SPHK2 (phospho T578) antibody
See all SPHK2 primary antibodies
DescriptionRabbit polyclonal to SPHK2 (phospho T578)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human SPHK2 (phospho T578) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9NRA0
- HeLa cell lysate treated with calyculin A or lambda phosphatase; Recombinant Human SPHK2 protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab215750 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 69 kDa.|
FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
Sequence similaritiesContains 1 DAGKc domain.
Cellular localizationCytoplasm. Membrane and Lysosome membrane.
- Information by UniProt
- SK 2 antibody
- Sphingosine kinase 2 antibody
- Sphingosine kinase type 2 antibody
Lanes 1-2 & 5-6 : anti-SPHK2 (N-terminal)
Lanes 3-4 & 7-8 : Anti-SPHK2 (phospho T578) antibody (ab215750) at 1/1000 dilution
Lanes 1 & 3 : recombinant Human SPHK2 protein
Lane 2 : recombinant Human SPHK2 protein treated with lambda phosphatase
Lane 4 : recombinant Human SPHK2 protein lambda phosphatase treated
Lane 5 : HeLa cell lysate calyculin A treated
Lanes 6 & 8 : HeLa cell lysate calyculin A and lambda phosphatase treated
Lane 7 : HeLa cell lysate calyculin A treated
Predicted band size: 69 kDa
ab215750 has not yet been referenced specifically in any publications.