Product nameAnti-SPHK2 (phospho T614) antibody
See all SPHK2 primary antibodies
DescriptionRabbit polyclonal to SPHK2 (phospho T614)
Specificityab111948 detects endogenous levels of SPHK2 only when phosphorylated at threonine 614.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phosphopeptide derived from Human SPHK2 around the phosphorylation site of threonine 614 (P-L-TP-P-R).
- Human Breast carcinoma tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
Note: PBS (without Mg2+ and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab111948 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab111948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
Sequence similaritiesContains 1 DAGKc domain.
Cellular localizationCytoplasm. Membrane and Lysosome membrane.
- Information by UniProt
- SK 2 antibody
- Sphingosine kinase 2 antibody
- Sphingosine kinase type 2 antibody
ab111948 has not yet been referenced specifically in any publications.