Anti-SPHK2 (phospho T614) antibody (ab111948)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPHK2 (phospho T614) antibody (ab111948)

    Key features and details

    • Rabbit polyclonal to SPHK2 (phospho T614)
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-SPHK2 (phospho T614) antibody
      See all SPHK2 primary antibodies

    • Description

      Rabbit polyclonal to SPHK2 (phospho T614)

    • Host species

      Rabbit

    • Specificity

      ab111948 detects endogenous levels of SPHK2 only when phosphorylated at threonine 614.

    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data

    • Immunogen

      Synthetic phosphopeptide derived from Human SPHK2 around the phosphorylation site of threonine 614 (P-L-TP-P-R).

    • Positive control

      • Human Breast carcinoma tissue.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab111948 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    All applications
    Mouse
    Rat
    Application Abreviews Notes
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPHK2 (phospho T614) antibody (ab111948)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPHK2 (phospho T614) antibody (ab111948)
      ab111948 at 1/50 dilution staining SPHK2 in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

    References (0)

    Publishing research using ab111948? Please let us know so that we can cite the reference in this datasheet.

    ab111948 has not yet been referenced specifically in any publications.

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