Overview

  • Product name
  • Description
    Rabbit polyclonal to SPHKAP
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant protein fragment, corresponding to a region within amino acids 1-219 of Human SPHKAP (UniProt ID: Q2M3C7).

  • Positive control
    • NT2D1, IMR32 and MCF7 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab155225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 186 kDa.

Target

  • Function
    Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.
  • Tissue specificity
    Highly expressed in heart. Both isoforms abundantly expressed in ventricle. Also expressed in spleen, ovary and brain.
  • Sequence similarities
    Belongs to the AKAP110 family.
  • Domain
    RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.
  • Cellular localization
    Cytoplasm. Colocalizes with SPHK1 in the cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • A-kinase anchor protein SPHKAP antibody
    • DKFZp781H143 antibody
    • DKFZp781J171 antibody
    • KIAA1678 antibody
    • MGC132614 antibody
    • MGC132616 antibody
    • SKIP antibody
    • Sphingosine kinase type 1-interacting protein antibody
    • SPHK1 (sphingosine kinase type 1) interacting protein antibody
    • SPHK1 interactor, AKAP domain containing antibody antibody
    • SPHK1-interacting protein antibody
    • SPHK1-interactor and AKAP domain-containing protein antibody
    • sphkap antibody
    • SPKAP_HUMAN antibody
    see all

References

ab155225 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Acetone

Dr. Maria Essers

Verified customer

Submitted May 29 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up