• Product name

  • Description

    Rabbit polyclonal to Spindly
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 475 and 525 of Human CCDC99 (NP_060255.2).

  • Positive control

    • HeLa whole cell lysate or 293T cell lysate


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab99344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 70 kDa.
IP Use at 2-5 µg/mg of lysate.


  • Function

    Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
  • Sequence similarities

    Belongs to the Spindly family.
  • Post-translational

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus. Cytoplasm > cytoskeleton > spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA409762 antibody
    • Arsenite related gene 1 protein antibody
    • Arsenite-related gene 1 protein antibody
    • CCDC 99 antibody
    • Ccdc99 antibody
    • Coiled coil domain containing 99 antibody
    • Coiled-coil domain-containing protein 99 antibody
    • FLJ20364 antibody
    • FLJ40690 antibody
    • hSpindly antibody
    • MGC124977 antibody
    • Protein Spindly antibody
    • rhabdomyosarcoma antigen MU RMS 40.4A antibody
    • Rhabdomyosarcoma antigen MU-RMS-40.4A antibody
    • Rrhabdomyosarcoma antigen protein MU RMS 40.4A antibody
    • SPDL1 antibody
    • SPDLY_HUMAN antibody
    • Spindle apparatus coiled coil domain containing protein 1 antibody
    • Spindle apparatus coiled coil protein 1 antibody
    • Spindly antibody
    • Spindly homolog antibody
    see all


  • All lanes : Anti-Spindly antibody (ab99344) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 70 kDa

    Exposure time: 3 minutes
  • 3µg ab99344 were used to precipitate CCDC99 from 1mg HeLa whole cell lysate.
    20% IP was loaded on gel and probed with ab99344 at 1µg/ml.
    Detection: chemiluminescence with exposure time of 10 seconds


ab99344 has not yet been referenced specifically in any publications.

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