Key features and details
- Rabbit polyclonal to Spindly
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Spindly antibody
See all Spindly primary antibodies
DescriptionRabbit polyclonal to Spindly
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee
Synthetic peptide, corresponding to a region between residue 475 and 525 of Human CCDC99 (NP_060255.2).
- HeLa whole cell lysate or 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab99344 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 70 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionRequired for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
Sequence similaritiesBelongs to the Spindly family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus. Cytoplasm > cytoskeleton > spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
- Information by UniProt
- AA409762 antibody
- Arsenite related gene 1 protein antibody
- Arsenite-related gene 1 protein antibody
All lanes : Anti-Spindly antibody (ab99344) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 70 kDa
Exposure time: 3 minutes
3µg ab99344 were used to precipitate CCDC99 from 1mg HeLa whole cell lysate.
20% IP was loaded on gel and probed with ab99344 at 1µg/ml.
Detection: chemiluminescence with exposure time of 10 seconds
ab99344 has not yet been referenced specifically in any publications.