Overview

  • Product name

    Anti-Spindly antibody - C-terminal
    See all Spindly primary antibodies
  • Description

    Rabbit Polyclonal to Spindly - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Fusion protein within Human Spindly (C terminal). The exact sequence is proprietary. (BC012568). The protein fusion partner is GST.
    Database link: Q96EA4

  • Positive control

    • Hela, Jurkat and mouse testis lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197682 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400 - 1/2000. Predicted molecular weight: 70 kDa.

Target

  • Function

    Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.
  • Sequence similarities

    Belongs to the Spindly family.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Nucleus. Cytoplasm > cytoskeleton > spindle pole. Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA409762 antibody
    • Arsenite related gene 1 protein antibody
    • Arsenite-related gene 1 protein antibody
    • CCDC 99 antibody
    • Ccdc99 antibody
    • Coiled coil domain containing 99 antibody
    • Coiled-coil domain-containing protein 99 antibody
    • FLJ20364 antibody
    • FLJ40690 antibody
    • hSpindly antibody
    • MGC124977 antibody
    • Protein Spindly antibody
    • rhabdomyosarcoma antigen MU RMS 40.4A antibody
    • Rhabdomyosarcoma antigen MU-RMS-40.4A antibody
    • Rrhabdomyosarcoma antigen protein MU RMS 40.4A antibody
    • SPDL1 antibody
    • SPDLY_HUMAN antibody
    • Spindle apparatus coiled coil domain containing protein 1 antibody
    • Spindle apparatus coiled coil protein 1 antibody
    • Spindly antibody
    • Spindly homolog antibody
    see all

Images

  • All lanes : Anti-Spindly antibody - C-terminal (ab197682) at 1/400 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : Mouse testis tissue lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 70 kDa


    Exposure time: 30 seconds


    8% SDS PAGE gel

References

ab197682 has not yet been referenced specifically in any publications.

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