Recombinant Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-SPINK1/P12 antibody [EPR17585-116]
See all SPINK1/P12 primary antibodies -
Description
Rabbit monoclonal [EPR17585-116] to SPINK1/P12 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human SPINK1/P12 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P00995 -
Positive control
- WB: Human pancreas lysate. IHC-P: Human pancreas and hepatocellular carcinoma tissues.
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General notes
This product was previously labelled as SPINK1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17585-116 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab207302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 8 kDa). |
Target
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Function
Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.
In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production. -
Involvement in disease
Pancreatitis, hereditary
Tropical calcific pancreatitis -
Sequence similarities
Contains 1 Kazal-like domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6690 Human
- Omim: 167790 Human
- SwissProt: P00995 Human
- Unigene: 407856 Human
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Alternative names
- ISK1_HUMAN antibody
- Pancreatic secretory trypsin inhibitor antibody
- Pancreatic serine protease inhibitor Kazal antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)](https://www.abcam.com/ps/products/207/ab207302/Images/ab207302-251704-anti-spink1-antibody-epr17585-116-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling SPINK1/P12 with ab207302 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human pancreas is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)](https://www.abcam.com/ps/products/207/ab207302/Images/ab207302-251703-anti-spink1-antibody-epr17585-116-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling SPINK1/P12 with ab207302 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on hepatocellular carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)](https://www.abcam.com/ps/products/207/ab207302/Images/ab207302-251705-anti-spink1-antibody-epr17585-116-western-blot.jpg)
Western blot - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)All lanes : Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302) at 1/5000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 8 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 minute; Lane 2: 3 minutes.
SPINK1/P12 is a pancreatic secretory trypsin inhibitor, uniquely expressed in pancreas. Human fetal kidney lysate is used as negative control.
SPINK1/P12 contains a signal peptide, thus observed with a MW of 11 kDa (PMCID: PMC2765331)
Datasheets and documents
References
ab207302 has not yet been referenced specifically in any publications.
