Recombinant Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)](https://www.abcam.com/ps/products/207/ab207302/Images/ab207302-251704-anti-spink1-antibody-epr17585-116-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17585-116] to SPINK1/P12
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-SPINK1/P12 antibody [EPR17585-116]
See all SPINK1/P12 primary antibodies -
Description
Rabbit monoclonal [EPR17585-116] to SPINK1/P12 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human SPINK1/P12 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P00995 -
Positive control
- WB: Human pancreas lysate. IHC-P: Human pancreas and hepatocellular carcinoma tissues.
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General notes
This product was previously labelled as SPINK1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17585-116 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab207302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. | |
| WB | 1/5000. Detects a band of approximately 11 kDa (predicted molecular weight: 8 kDa). |
Target
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Function
Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.
In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production. -
Involvement in disease
Pancreatitis, hereditary
Tropical calcific pancreatitis -
Sequence similarities
Contains 1 Kazal-like domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6690 Human
- Omim: 167790 Human
- SwissProt: P00995 Human
- Unigene: 407856 Human
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Alternative names
- ISK1_HUMAN antibody
- Pancreatic secretory trypsin inhibitor antibody
- Pancreatic serine protease inhibitor Kazal antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling SPINK1/P12 with ab207302 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human pancreas is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)](https://www.abcam.com/ps/products/207/ab207302/Images/ab207302-251703-anti-spink1-antibody-epr17585-116-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling SPINK1/P12 with ab207302 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on hepatocellular carcinoma is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Western blot - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)](https://www.abcam.com/ps/products/207/ab207302/Images/ab207302-251705-anti-spink1-antibody-epr17585-116-western-blot.jpg)
Western blot - Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)All lanes : Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302) at 1/5000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 8 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 minute; Lane 2: 3 minutes.
SPINK1/P12 is a pancreatic secretory trypsin inhibitor, uniquely expressed in pancreas. Human fetal kidney lysate is used as negative control.
SPINK1/P12 contains a signal peptide, thus observed with a MW of 11 kDa (PMCID: PMC2765331)
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![Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)](https://www.abcam.com/ps/products/207/ab207302/Images/ab207302-4-benefits-of-recombinant-antibodies.png)
Anti-SPINK1/P12 antibody [EPR17585-116] (ab207302)
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab207302 has not yet been referenced specifically in any publications.
