Recombinant Anti-SPINK1/P12 antibody [SP166] (ab183347)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP166] to SPINK1/P12
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SPINK1/P12 antibody [SP166]
See all SPINK1/P12 primary antibodies -
Description
Rabbit monoclonal [SP166] to SPINK1/P12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human SPINK1/P12 aa 1 to the C-terminus. Without signal peptide.
Database link: P00995 -
Positive control
- Human prostate adenocarcinoma tissue; Human pancreas lysate
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General notes
Cow: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP166 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100. Predicted molecular weight: 8 kDa.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/100. Predicted molecular weight: 8 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:7142173). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.
In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production. -
Involvement in disease
Pancreatitis, hereditary
Tropical calcific pancreatitis -
Sequence similarities
Contains 1 Kazal-like domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6690 Human
- Omim: 167790 Human
- SwissProt: P00995 Human
- Unigene: 407856 Human
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Alternative names
- ISK1_HUMAN antibody
- Pancreatic secretory trypsin inhibitor antibody
- Pancreatic serine protease inhibitor Kazal antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183347 has not yet been referenced specifically in any publications.