Anti-SPINK5/LEKTI antibody (ab246890)
Key features and details
- Rabbit polyclonal to SPINK5/LEKTI
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPINK5/LEKTI antibody
See all SPINK5/LEKTI primary antibodies -
Description
Rabbit polyclonal to SPINK5/LEKTI -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human SPINK5/LEKTI aa 150-300.
Database link: Q9NQ38 -
Positive control
- IHC-P: Human tonsil, skin, uterine cervix and kidney tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab246890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin. -
Tissue specificity
Highly expressed in the thymus and stratum corneum. Also found in the oral mucosa, parathyroid gland, Bartholin's glands, tonsils, and vaginal epithelium. Very low levels are detected in lung, kidney, and prostate. -
Involvement in disease
Defects in SPINK5 are the cause of Netherton syndrome (NETH) [MIM:256500]. NETH is an autosomal recessive congenital ichthyosis associated with hair shaft abnormalities and anomalies of the immune system. Typical features are ichthyosis linearis circumflexa, ichthyosiform erythroderma, trichorrhexis invaginata (bamboo hair), atopic dermatitis, and hayfever. High postnatal mortality is due to failure to thrive, infections and hypernatremic dehydration. -
Sequence similarities
Contains 15 Kazal-like domains. -
Domain
Contains at least one active inhibitory domain for trypsin (domain 6). -
Post-translational
modificationsProteolytically processed by furin in individual domains (D1, D5, D6, D8 through D11, and D9 through D15) exhibiting various inhibitory potentials for multiple proteases. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 11005 Human
- Omim: 605010 Human
- SwissProt: Q9NQ38 Human
- Unigene: 331555 Human
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Alternative names
- Hemofiltrate peptide HF7665 antibody
- ISK5_HUMAN antibody
- LEKTI antibody
see all
Images
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PFA-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for SPINK5/LEKTI (green) using ab246890 at 4 µg/ml in ICC/IF.
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Paraffin-embedded human kidney tissue stained for SPINK5/LEKTI using ab246890 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human uterine cervix tissue stained for SPINK5/LEKTI using ab246890 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human skin tissue stained for SPINK5/LEKTI using ab246890 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human tonsil tissue stained for SPINK5/LEKTI using ab246890 at 1/200 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab246890 has not yet been referenced specifically in any publications.