Overview

  • Product name

    Anti-SPINK5/LEKTI antibody [EPR8777]
    See all SPINK5/LEKTI primary antibodies
  • Description

    Rabbit monoclonal [EPR8777] to SPINK5/LEKTI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human SPINK5/LEKTI aa 150-250.
    Database link: Q9NQ38-1

  • Positive control

    • Fetal heart; HeLa whole cell lysate (ab150035); Mouse heart; Rat heart.
  • General notes

     

     

     This product was previously labelled as SPINK5

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8777
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 121 kDa.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin.
    • Tissue specificity

      Highly expressed in the thymus and stratum corneum. Also found in the oral mucosa, parathyroid gland, Bartholin's glands, tonsils, and vaginal epithelium. Very low levels are detected in lung, kidney, and prostate.
    • Involvement in disease

      Defects in SPINK5 are the cause of Netherton syndrome (NETH) [MIM:256500]. NETH is an autosomal recessive congenital ichthyosis associated with hair shaft abnormalities and anomalies of the immune system. Typical features are ichthyosis linearis circumflexa, ichthyosiform erythroderma, trichorrhexis invaginata (bamboo hair), atopic dermatitis, and hayfever. High postnatal mortality is due to failure to thrive, infections and hypernatremic dehydration.
    • Sequence similarities

      Contains 15 Kazal-like domains.
    • Domain

      Contains at least one active inhibitory domain for trypsin (domain 6).
    • Post-translational
      modifications

      Proteolytically processed by furin in individual domains (D1, D5, D6, D8 through D11, and D9 through D15) exhibiting various inhibitory potentials for multiple proteases.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Hemofiltrate peptide HF7665 antibody
      • ISK5_HUMAN antibody
      • LEKTI antibody
      • Lympho-epithelial Kazal-type-related inhibitor antibody
      • Lymphoepithelial Kazal type related inhibitor antibody
      • NETS antibody
      • NS antibody
      • Serine peptidase inhibitor Kazal type 5 antibody
      • SPINK5 antibody
      • VAKTI antibody
      see all

    Images

    • All lanes : Anti-SPINK5/LEKTI antibody [EPR8777] (ab138511) at 1/5000 dilution (purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse heart lysates
      Lane 3 : Rat heart lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 121 kDa



      Blocking and diluting buffer : 5% NFDM/TBST
    • Immunofluorescent analysis of HeLa cells labeling Spink5/LEKTI with ab138511 at 1:500 dilution.  

    • All lanes : Anti-SPINK5/LEKTI antibody [EPR8777] (ab138511) at 1/1000 dilution

      Lane 1 : Fetal heart cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 121 kDa

    References

    ab138511 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab138511.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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