Recombinant
RabMAb

Recombinant Anti-SPINK5/LEKTI antibody [EPR8777] - BSA and Azide free (ab232501)

Overview

  • Product name

    Anti-SPINK5/LEKTI antibody [EPR8777] - BSA and Azide free
    See all SPINK5/LEKTI primary antibodies
  • Description

    Rabbit monoclonal [EPR8777] to SPINK5/LEKTI - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SPINK5/LEKTI aa 150-250. The exact sequence is proprietary.
    Database link: Q9NQ38-1

  • Positive control

    • WB: HeLa cell lysates; Mouse and rat heart lysates.
  • General notes

    ab232501 is the carrier-free version of ab138511 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232501 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as SPINK5

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8777
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 121 kDa.

Target

  • Function

    Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin.
  • Tissue specificity

    Highly expressed in the thymus and stratum corneum. Also found in the oral mucosa, parathyroid gland, Bartholin's glands, tonsils, and vaginal epithelium. Very low levels are detected in lung, kidney, and prostate.
  • Involvement in disease

    Defects in SPINK5 are the cause of Netherton syndrome (NETH) [MIM:256500]. NETH is an autosomal recessive congenital ichthyosis associated with hair shaft abnormalities and anomalies of the immune system. Typical features are ichthyosis linearis circumflexa, ichthyosiform erythroderma, trichorrhexis invaginata (bamboo hair), atopic dermatitis, and hayfever. High postnatal mortality is due to failure to thrive, infections and hypernatremic dehydration.
  • Sequence similarities

    Contains 15 Kazal-like domains.
  • Domain

    Contains at least one active inhibitory domain for trypsin (domain 6).
  • Post-translational
    modifications

    Proteolytically processed by furin in individual domains (D1, D5, D6, D8 through D11, and D9 through D15) exhibiting various inhibitory potentials for multiple proteases.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Hemofiltrate peptide HF7665 antibody
    • ISK5_HUMAN antibody
    • LEKTI antibody
    • Lympho-epithelial Kazal-type-related inhibitor antibody
    • Lymphoepithelial Kazal type related inhibitor antibody
    • NETS antibody
    • NS antibody
    • Serine peptidase inhibitor Kazal type 5 antibody
    • SPINK5 antibody
    • VAKTI antibody
    see all

Images

  • Immunofluorescent analysis of HeLa cells labeling Spink5/LEKTI with ab138511 at 1:500 dilution.  

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138511).

  • All lanes : Anti-SPINK5/LEKTI antibody [EPR8777] (ab138511) at 1/5000 dilution

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse heart lysates
    Lane 3 : Rat heart lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 121 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab138511).

References

ab232501 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232501.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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