Overview

  • Product name

    Anti-Spinophilin/Neurabin 2 antibody
    See all Spinophilin/Neurabin 2 primary antibodies
  • Description

    Rabbit polyclonal to Spinophilin/Neurabin 2
  • Host species

    Rabbit
  • Specificity

    Specific for rat spinophilin at 120kD. Also recognizes a band at ~95 kDa which is suspected to be a cleavage product of neurabin 2.
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog, Human, Chimpanzee
  • Immunogen

    Fusion protein, corresponding to internal sequence amino acids 286-390 of Rat brain Neurabin 2.

  • Positive control

    • Rat brain microsomal preparation.

Properties

Applications

Our Abpromise guarantee covers the use of ab18561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notes
    IP: 4 µg immunoprecipitates Neurabin 2 from 500 µg of rat brain microsomal preparation.
    WB: 0.25 - 1 µg/ml detected Neurabin 2 in 20 µg of a rat brain microsomal preparation. Detects a band of approximately 120 kDa (predicted molecular weight: 89 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity). Required for hepatocyte growth factor (HGF)-induced cell migration.
    • Sequence similarities

      Contains 1 PDZ (DHR) domain.
    • Post-translational
      modifications

      Stimulation of D1 (but not D2) dopamine receptors induces Ser-94 phosphorylation. Dephosphorylation of Ser-94 is mediated mainly by PP1 and to a lesser extent by PP2A. Phosphorylation of spinophilin disrupts its association with F-actin, but does not affect its binding to PP1.
    • Cellular localization

      Cytoplasm > cytoskeleton. Nucleus. Cell projection > dendritic spine. Cell junction > synapse. Cell junction > adherens junction. Cytoplasm. Cell membrane. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > ruffle membrane. Enriched at synapse and cadherin-based cell-cell adhesion sites. In neurons, both cytosolic and membrane-associated, and highly enriched in the post-synaptic density apposed to exitatory synapses. Colocalizes with PPP1R2 at actin-rich adherens junctions in epithelial cells and in dendritic spines (By similarity). Accumulates in the lamellipodium, filopodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ30345 antibody
      • NEB2_HUMAN antibody
      • Neurabin II antibody
      • Neurabin-2 antibody
      • Neurabin-II antibody
      • Neurabin2 antibody
      • NeurabinII antibody
      • Neural tissue specific F actin binding protein II antibody
      • p130 antibody
      • PP1bp134 antibody
      • PPP1R6 antibody
      • PPP1R9 antibody
      • Ppp1r9b antibody
      • Protein phosphatase 1 regulatory subunit 9B antibody
      • SPINO antibody
      • Spinophilin antibody
      see all

    References

    This product has been referenced in:

    • Liu M  et al. Maternal exposure to swainsonine impaired the early postnatal development of mouse dentate gyrus of offspring. Neurochem Int 129:104511 (2019). Read more (PubMed: 31348968) »
    • Pitt J  et al. Neuroprotective astrocyte-derived insulin/insulin-like growth factor 1 stimulates endocytic processing and extracellular release of neuron-bound Aß oligomers. Mol Biol Cell 28:2623-2636 (2017). Read more (PubMed: 28963439) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you very much for your time and providing this further information. Your Abreview is really appreciated.
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    I wish you good look with your research. If you need any more information please do not hesitate to contact us.

    Read More
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Apteronotus leptorhynchus Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% Triton X-100
    Blocking step
    3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Dec 03 2012

    Application
    Western blot
    Sample
    Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount
    50 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (4-15 %)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Abcam user community

    Verified customer

    Submitted Nov 26 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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