• Product name
    Anti-Spinophilin/Neurabin 2 antibody
    See all Spinophilin/Neurabin 2 primary antibodies
  • Description
    Rabbit polyclonal to Spinophilin/Neurabin 2
  • Host species
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to C terminal amino acids 800-817 of Rat Neurabin 2

  • Positive control
    • Mouse brain and rat brain extracts (S1 fraction), Madin-Darby kidney (MDCK) cells.



Our Abpromise guarantee covers the use of ab50184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 89 kDa.
IP Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 - 20 µg/ml.


  • Function
    Seems to act as a scaffold protein in multiple signaling pathways. Modulates excitatory synaptic transmission and dendritic spine morphology. Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. Believed to target protein phosphatase 1/PP1 to dendritic spines, which are rich in F-actin, and regulates its specificity toward ion channels and other substrates, such as AMPA-type and NMDA-type glutamate receptors. Plays a role in regulation of G-protein coupled receptor signaling, including dopamine D2 receptors and alpha-adrenergic receptors. May establish a signaling complex for dopaminergic neurotransmission through D2 receptors by linking receptors downstream signaling molecules and the actin cytoskeleton. Binds to ADRA1B and RGS2 and mediates regulation of ADRA1B signaling. May confer to Rac signaling specificity by binding to both, RacGEFs and Rac effector proteins. Probably regulates p70 S6 kinase activity by forming a complex with TIAM1 (By similarity). Required for hepatocyte growth factor (HGF)-induced cell migration.
  • Sequence similarities
    Contains 1 PDZ (DHR) domain.
  • Post-translational
    Stimulation of D1 (but not D2) dopamine receptors induces Ser-94 phosphorylation. Dephosphorylation of Ser-94 is mediated mainly by PP1 and to a lesser extent by PP2A. Phosphorylation of spinophilin disrupts its association with F-actin, but does not affect its binding to PP1.
  • Cellular localization
    Cytoplasm > cytoskeleton. Nucleus. Cell projection > dendritic spine. Cell junction > synapse. Cell junction > adherens junction. Cytoplasm. Cell membrane. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > ruffle membrane. Enriched at synapse and cadherin-based cell-cell adhesion sites. In neurons, both cytosolic and membrane-associated, and highly enriched in the post-synaptic density apposed to exitatory synapses. Colocalizes with PPP1R2 at actin-rich adherens junctions in epithelial cells and in dendritic spines (By similarity). Accumulates in the lamellipodium, filopodium and ruffle membrane in response to hepatocyte growth factor (HGF) treatment.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ30345 antibody
    • NEB2_HUMAN antibody
    • Neurabin II antibody
    • Neurabin-2 antibody
    • Neurabin-II antibody
    • Neurabin2 antibody
    • NeurabinII antibody
    • Neural tissue specific F actin binding protein II antibody
    • p130 antibody
    • PP1bp134 antibody
    • PPP1R6 antibody
    • PPP1R9 antibody
    • Ppp1r9b antibody
    • Protein phosphatase 1 regulatory subunit 9B antibody
    • SPINO antibody
    • Spinophilin antibody
    see all


This product has been referenced in:
  • Bocarsly ME  et al. Obesity diminishes synaptic markers, alters microglial morphology, and impairs cognitive function. Proc Natl Acad Sci U S A 112:15731-6 (2015). Read more (PubMed: 26644559) »
  • Fu X  et al. Doublecortin (Dcx) family proteins regulate filamentous actin structure in developing neurons. J Neurosci 33:709-21 (2013). Read more (PubMed: 23303949) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab50184.
Please use the links above to contact us or submit feedback about this product.


Sign up