Key features and details
- 5-HT and D2-like receptor antagonist
- CAS Number: 2022-29-9
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
Product nameSpiperone hydrochloride, 5-HT and D2-like receptor antagonist
Description5-HT and D2-like receptor antagonist
5-HT and D2-like receptor antagonist. (IC50 values are 1.6 and 15 nM at 5-HT2 and 5-HT1C receptors, respectively. Ki values are 0.06 ( D2), 0.6 (D3), 0.08 (D4), ~ 350 (D1) and ~ 3500 nM (D5)). Potently enhances intracellular Ca2+ levels. Additionally activates Ca2+-activated Cl- channels (CaCC) to induce Cl- secretion.
Chemical name8-[4-(4-Fluorophenyl)-4-oxobutyl]-1-phenyl-1,3,8-triazaspiro[4,5]decan-4-one hydrochloride
Storage instructionsStore at Room Temperature. The product can be stored for up to 12 months.
Solubility overviewSoluble in DMSO to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
2D chemical structure image of ab120549, Spiperone hydrochloride, 5-HT and D2-like receptor antagonist
Cabergoline exerted neuroprotective effect via D2 receptor-mediated mechanism.
(Panel B) Spiperone inhibited neuroprotection by cabergoline. Spiperone (10 µM) was applied 20 mins before cabergoline (10 µM) treatment, followed by MTT assay. The data represent mean ± SD (n = 6–12). ***P<0.001 vs. - H2O2 - Caber - spiperone, †††P<0.001 vs. + H2O2 - Caber - spiperone (three-way ANOVA).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120549 has been referenced in 2 publications.
- Ward AH et al. Intravitreally-administered dopamine D2-like (and D4), but not D1-like, receptor agonists reduce form-deprivation myopia in tree shrews. Vis Neurosci 34:E003 (2017). PubMed: 28304244
- Odaka H et al. Cabergoline, dopamine D2 receptor agonist, prevents neuronal cell death under oxidative stress via reducing excitotoxicity. PLoS One 9:e99271 (2014). PubMed: 24914776