Product nameAnti-Spir-1 antibody
DescriptionRabbit polyclonal to Spir-1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- RT4, U-251 MG, Human Plasma and Human Liver lysates, Human lateral ventricle tissue
General notesStore product undiluted. For continuous use, store at 2-8°C for one-two days. Working dilution samples should be discarded if not used within 12 hours. The antibody solution should be gently mixed before use.
This antibody is mono-specific.
Previously labelled as SPIRE1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab130403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 86 kDa.|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionActs as a actin nucleation factor, remains associated with the slow-growing pointed end of the new filament. Involved in vesicle transport processes providing a novel link between actin organization and intracellular transport.
Sequence similaritiesBelongs to the spire family.
Contains 1 KIND domain.
Contains 2 WH2 domains.
DomainBinds to actin monomers via the WH2 domain.
The Spir-box targets binding to intracellular membrane structures.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > perinuclear region. Punctate spots in perinuclear region and cytoplasm, co-localised with Rab11.
- Information by UniProt
- KIAA1135 antibody
- MGC150621 antibody
- MGC150622 antibody
All lanes : Anti-Spir-1 antibody (ab130403) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U-251 MG cell lysate
Lane 3 : Human Plasma lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Predicted band size: 86 kDa
Immunohistochemical analysis of paraffin embedded human lateral ventricle tissue labelled with ab130403 antibody at 1/500 dilution.
ab130403 has not yet been referenced specifically in any publications.