Overview

  • Product name

    Anti-sPLA2-X antibody [EPR11202]
    See all sPLA2-X primary antibodies
  • Description

    Rabbit monoclonal [EPR11202] to sPLA2-X
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human sPLA2-X.
    Database link: O15496

  • Positive control

    • TT, A549, MCF7 and Caco 2 cell lysates; Human pancreas and tonsil tissues; permeabilized A549 cells.
  • General notes

     This product was previously labelled as Phospholipase A2 X

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab166634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids. Prefers phosphatidylethanolamine and phosphatidylcholine liposomes to those of phosphatidylserine.
    • Tissue specificity

      Found in spleen, thymus, peripheral blood leukocytes, pancreas, lung, and colon.
    • Sequence similarities

      Belongs to the phospholipase A2 family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Group 10 secretory phospholipase A2 antibody
      • Group X secretory phospholipase A2 antibody
      • GX sPLA2 antibody
      • GXPLA2 antibody
      • GXSPLA2 antibody
      • PA2GX_HUMAN antibody
      • Phosphatidylcholine 2 acylhydrolase GX antibody
      • Phosphatidylcholine 2-acylhydrolase 10 antibody
      • Phospholipase A2 group X antibody
      • PLA2G10 antibody
      • SPLA2 antibody
      • sPLA2-X antibody
      • sPLA2X antibody
      see all

    Images

    • All lanes : Anti-sPLA2-X antibody [EPR11202] (ab166634) at 1/1000 dilution

      Lane 1 : TT cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 17 kDa

    • Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling sPLA2-X with ab166634 at a 1/100 dilution.

    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling sPLA2-X with ab166634 at a 1/100 dilution.

    • Flow cytometric analysis of permeabilized A549 cells using ab166634 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:

    • Zhang D  et al. The inhibitory effect of saPLI?, a snake sourced PLA2 inhibitor on carrageenan-induced inflammation in mice. Toxicon 151:89-95 (2018). Read more (PubMed: 30003915) »
    • Nolin JD  et al. Secreted PLA2 group X orchestrates innate and adaptive immune responses to inhaled allergen. JCI Insight 2:N/A (2017). WB ; Mouse . Read more (PubMed: 29093264) »
    See all 2 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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