Overview

  • Product name

  • Description

    Rabbit polyclonal to SPLASH
  • Host species

    Rabbit
  • Specificity

    This antibody is specific for SPLASH.

  • Tested applications

    Suitable for: ICC/IF, Functional Studies, ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human SPLASH.

  • General notes

     This product was previously labelled as Phospholipase A2 IID

     

Properties

Applications

Our Abpromise guarantee covers the use of ab47118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50. PubMed: 22240557
Functional Studies Use a concentration of 2 µg/ml. PubMed: 22240557
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa.

Target

  • Function

    PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. L-alpha-1-palmitoyl-2-linoleoyl phosphatidylethanolamine is more efficiently hydrolyzed than the other phospholipids examined.
  • Tissue specificity

    Broadly expressed.
  • Sequence similarities

    Belongs to the phospholipase A2 family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2IID antibody
    • EC 3.1.1.4 antibody
    • GIID sPLA2 antibody
    • Group IID secretory phospholipase A2 antibody
    • Group IID secretory phospholipase A2 precursor antibody
    • PA2GD_HUMAN antibody
    • Phosphatidylcholine 2 acylhydrolase GIID antibody
    • Phosphatidylcholine 2-acylhydrolase 2D antibody
    • Phospholipase A2 group IID antibody
    • Pla2g2d antibody
    • PLA2IID antibody
    • Secretory phospholipase A2s antibody
    • Secretory type PLA stroma associated homolog antibody
    • Secretory-type PLA antibody
    • sPLA antibody
    • sPLA2-IID antibody
    • sPLA2S antibody
    • SPLASH antibody
    • stroma-associated homolog antibody
    see all

References

This product has been referenced in:

  • Mosher AA  et al. Regulation of sPLA2-IID in Human Decidua: Insights Into the Complexity of the Prostaglandin Pathway in Labor. Reprod Sci N/A:N/A (2014). Human . Read more (PubMed: 24440994) »
  • Li K  et al. Secretory Phospholipase A2 Group IID Is Involved in Progesterone-Induced Acrosomal Exocytosis of Human Spermatozoa. J Androl 33:975-83 (2012). WB, ICC/IF, Functional Studies ; Human . Read more (PubMed: 22240557) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
ELISA
Sample
Human Recombinant protein (PLA2G2D(h)(21-145)-hIgG1Fc)
Specification
PLA2G2D(h)(21-145)-hIgG1Fc
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Type
Sandwich (Capture)

Abcam user community

Verified customer

Submitted Oct 04 2018

Application
ELISA
Sample
Mouse Recombinant protein (PLA2G2D(m)(20-144)-hIgG1Fc)
Specification
PLA2G2D(m)(20-144)-hIgG1Fc
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Type
Sandwich (Capture)

Abcam user community

Verified customer

Submitted Oct 04 2018

Application
ELISA
Sample
Cynomolgus monkey Purified protein ([PLA2G2D(cy)(21-145)]-[hu IgG1 Fc])
Specification
[PLA2G2D(cy)(21-145)]-[hu IgG1 Fc]
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Type
Sandwich (Capture)

Abcam user community

Verified customer

Submitted Oct 03 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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