Key features and details
- Rabbit polyclonal to splicing factor 1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-splicing factor 1 antibody
See all splicing factor 1 primary antibodies
DescriptionRabbit polyclonal to splicing factor 1
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- Human adrenal tissue; COLO205 cell extract; HeLa cells
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, 49% PBS
PBS (without Mg2+, Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab189274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/500.|
|WB||1/500 - 1/1000. Predicted molecular weight: 68 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
RelevanceSF1 contains 1 KH domain, 1 CCHC type zinc finger, a nuclear transport domain and glutamine and proline rich regions. It is necessary for the ATP dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5' UACUAAC 3' of the pre mRNA. It may act as transcription repressor. There are 6 named isoforms produced by alternative splicing.
- BBP antibody
- D11S636 antibody
- Mammalian branch point binding protein mBBP antibody
All lanes : Anti-splicing factor 1 antibody (ab189274) at 1/500 dilution
Lane 1 : COLO205 cell extract
Lane 2 : COLO205 cell extract with synthesized peptide
Predicted band size: 68 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adrenal tissue, labeling splicing factor 1 with ab189274 at 10 µg/ml.
Immunofluorescence analysis of HeLa cells, labeling splicing factor 1 with ab189274 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.
ab189274 has not yet been referenced specifically in any publications.