Key features and details
- Rabbit polyclonal to splicing factor 1
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-splicing factor 1 antibody
See all splicing factor 1 primary antibodies
DescriptionRabbit polyclonal to splicing factor 1
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- IHC-P: Human urinary bladder tissue. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab224106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
RelevanceSF1 contains 1 KH domain, 1 CCHC type zinc finger, a nuclear transport domain and glutamine and proline rich regions. It is necessary for the ATP dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5' UACUAAC 3' of the pre mRNA. It may act as transcription repressor. There are 6 named isoforms produced by alternative splicing.
- BBP antibody
- D11S636 antibody
- Mammalian branch point binding protein mBBP antibody
ab224106 has not yet been referenced specifically in any publications.