Overview

  • Product name

    Anti-splicing factor 1 antibody
    See all splicing factor 1 primary antibodies
  • Description

    Rabbit polyclonal to splicing factor 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human splicing factor 1 (internal sequence). The exact sequence is proprietary.
    Database link: Q15637

  • Positive control

    • WB: Raji whole cell lysate. ICC/IF: A-375 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 68 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    SF1 contains 1 KH domain, 1 CCHC type zinc finger, a nuclear transport domain and glutamine and proline rich regions. It is necessary for the ATP dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5' UACUAAC 3' of the pre mRNA. It may act as transcription repressor. There are 6 named isoforms produced by alternative splicing.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • BBP antibody
    • D11S636 antibody
    • Mammalian branch point binding protein mBBP antibody
    • OTTHUMP00000069749 antibody
    • OTTHUMP00000069750 antibody
    • OTTHUMP00000069752 antibody
    • Splicing factor 1 antibody
    • Transcription factor ZFM1 antibody
    • ZFM1 antibody
    • Zinc finger gene in MEN1 locus antibody
    • Zinc finger protein 162 antibody
    • ZNF162 antibody
    see all

Images

  • Anti-splicing factor 1 antibody (ab228535) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 68 kDa



    7.5% SDS-PAGE

  • A-375 (human malignant melanoma cell line) cells stained for splicing factor 1 (green) using ab228535 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

References

ab228535 has not yet been referenced specifically in any publications.

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