Key features and details
- Rabbit polyclonal to splicing factor 1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-splicing factor 1 antibody
See all splicing factor 1 primary antibodies
DescriptionRabbit polyclonal to splicing factor 1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human splicing factor 1 (internal sequence). The exact sequence is proprietary.
Database link: Q15637
- WB: Raji whole cell lysate. ICC/IF: A-375 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228535 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 68 kDa.|
|ICC/IF||1/100 - 1/1000.|
RelevanceSF1 contains 1 KH domain, 1 CCHC type zinc finger, a nuclear transport domain and glutamine and proline rich regions. It is necessary for the ATP dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5' UACUAAC 3' of the pre mRNA. It may act as transcription repressor. There are 6 named isoforms produced by alternative splicing.
- BBP antibody
- D11S636 antibody
- Mammalian branch point binding protein mBBP antibody
Anti-splicing factor 1 antibody (ab228535) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 68 kDa
A-375 (human malignant melanoma cell line) cells stained for splicing factor 1 (green) using ab228535 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.
Blue: Hoechst 33342 staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228535 has not yet been referenced specifically in any publications.