Overview

  • Product name

    Splitomicin, Sir2P inhibitor
  • Description

    Sir2P inhibitor
  • Biological description

    Silent information regulator 2P (Sir2P)/ SIRT1 inhibitor (IC50 = 60 μM). Able to inhibit NAD+-dependent histone deacetylase (HDAC) activity of the Sir2 protein in vitro. Active in vivo.
  • Purity

    > 97%
  • CAS Number

    5690-03-9
  • Chemical structure

    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab141120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab66705 staining PAI1 in MCF-7 cells treated with splitomicin (ab141120), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of splitomicin, as described in literature.
    The cells were incubated at 37°C for 48 hours in media containing different concentrations of ab141120 (splitomicin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab66705 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue

References

This product has been referenced in:

  • Beier UH  et al. Sirtuin-1 targeting promotes Foxp3+ T-regulatory cell function and prolongs allograft survival. Mol Cell Biol 31:1022-9 (2011). Read more (PubMed: 21199917) »
  • Kang MR  et al. Reciprocal roles of SIRT1 and SKIP in the regulation of RAR activity: implication in the retinoic acid-induced neuronal differentiation of P19 cells. Nucleic Acids Res 38:822-31 (2010). Read more (PubMed: 19934264) »
  • Hirao M  et al. Identification of selective inhibitors of NAD+-dependent deacetylases using phenotypic screens in yeast. J Biol Chem 278:52773-82 (2003). Read more (PubMed: 14534292) »
  • Bedalov A  et al. Identification of a small molecule inhibitor of Sir2p. Proc Natl Acad Sci U S A 98:15113-8 (2001). Read more (PubMed: 11752457) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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