Anti-SPNS1 antibody (ab59971)
Key features and details
- Rabbit polyclonal to SPNS1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SPNS1 antibody -
Description
Rabbit polyclonal to SPNS1 -
Host species
Rabbit -
Specificity
This antibody reacts specifically with human 56 kDa SPNS1 protein. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide derived from N terminal region of human SPNS1 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (3) |
Use a concentration of 5 µg/ml.
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WB |
1/500 - 1/2000. Predicted molecular weight: 56 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
WB
1/500 - 1/2000. Predicted molecular weight: 56 kDa. |
Target
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Function
Sphingolipid transporter (By similarity). May be involved in necrotic or autophagic cell death. -
Sequence similarities
Belongs to the major facilitator superfamily. Spinster family. -
Cellular localization
Mitochondrion inner membrane. Colocalizes with SDHB. - Information by UniProt
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Database links
- Entrez Gene: 83985 Human
- Entrez Gene: 73658 Mouse
- SwissProt: Q9H2V7 Human
- SwissProt: Q8R0G7 Mouse
- Unigene: 617449 Human
- Unigene: 11112 Mouse
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Alternative names
- HSpin1 antibody
- LAT antibody
- nrs antibody
see all
Images
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All lanes : Anti-SPNS1 antibody (ab59971) at 1/1000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 49 kDa (possible isoform) -
ICC/IF image of ab59971 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59971, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab59971 has been referenced in 1 publication.
- Guo H et al. Multi-omics analyses reveal that HIV-1 alters CD4+ T cell immunometabolism to fuel virus replication. Nat Immunol 22:423-433 (2021). PubMed: 33767427