Anti-SPOCD1 antibody (ab122188)
Key features and details
- Rabbit polyclonal to SPOCD1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SPOCD1 antibody -
Description
Rabbit polyclonal to SPOCD1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to amino acids 995-1085 (SPLLSPGLEV THSSLLLAVL LPKEGLPDTA GSSPWLGKVQ KMVSFNSKVE KRYYQPDDRR PNVPLKGTPP PGGAWQQSQG RGSIAPRGIS A) of Human SPOCD1.
-
Positive control
- IHC-P: Human testis tissue; WB: U-251 MG and Human Plasma lysates; ICC/IF: A431 cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
|
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
Notes |
---|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
-
Sequence similarities
Contains 1 SPOC domain.
Contains 1 TFIIS central domain. - Information by UniProt
-
Database links
- Entrez Gene: 90853 Human
- SwissProt: Q6ZMY3 Human
- Unigene: 62604 Human
-
Alternative names
- FLJ25348 antibody
- RP11-84A19.1 antibody
- SPOC domain-containing protein 1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunocytochemistry/immunofluorescence analysis of A431 cells labelling SPOCD1 with ab122188 shows positive staining in the nucleus and nucleoli.
-
All lanes : Anti-SPOCD1 antibody (ab122188) at 0.4 µg/ml
Lane 1 : RT4 (human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma (IgG/HSA depleted) lysate -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling SPOCD1 with ab122188 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab122188 has been referenced in 2 publications.
- Liang J et al. SPOCD1 promotes cell proliferation and inhibits cell apoptosis in human osteosarcoma. Mol Med Rep 17:3218-3225 (2018). PubMed: 29257309
- Wang Y et al. Differential expression profile analysis of lncRNA UCA1α regulated mRNAs in bladder cancer. J Cell Biochem 119:1841-1854 (2018). PubMed: 28815726