Overview

  • Product name

  • Description

    Rabbit polyclonal to SPOCK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human SPOCK2 aa 100-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    VCIAQGYQRAM CISRKKLEHR IKQPTVKLHG NKDSICKPCH MAQLASVCGS


    Database link: Q92563

  • Positive control

    • HeLa cell lysate; Rat brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab217044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/1000. Predicted molecular weight: 47 kDa.

Target

  • Function

    May participate in diverse steps of neurogenesis. Binds calcium.
  • Tissue specificity

    Highly expressed in brain. Also found in lung and testis.
  • Sequence similarities

    Contains 1 Kazal-like domain.
    Contains 1 thyroglobulin type-1 domain.
  • Post-translational
    modifications

    Contains chondroitin sulfate and heparan sulfate O-linked oligosaccharides.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • and Kazal-like domains proteoglycan 2 antibody
    • CWCV antibody
    • FLJ97039 antibody
    • KIAA0275 antibody
    • SPARC/osteonectin antibody
    • Sparc/osteonectin cwcv and kazal like domains proteoglycan (testican) 2 antibody
    • Sparc/osteonectin cwcv and kazal like domains proteoglycan 2 antibody
    • SPOCK 2 antibody
    • Spock2 antibody
    • Testican 2 antibody
    • Testican-2 antibody
    • Testican2 antibody
    • TICN 2 antibody
    • TICN2 antibody
    • TICN2_HUMAN antibody
    see all

Images

  • Anti-SPOCK2 antibody (ab217044) at 1/300 dilution + HeLa cell lysate

    Secondary
    Anti-rabbit antibody at 1/10000 dilution

    Predicted band size: 47 kDa

  • Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded Rat brain tissue labeling SPOCK2 with ab217044 at 1/200 dilution followed by a conjugated secondary and DAB staining.

References

ab217044 has not yet been referenced specifically in any publications.

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