• Product name
  • Description
    Chicken polyclonal to SPON1
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 549-807 of SPON1.



Our Abpromise guarantee covers the use of ab14271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 100.9 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 24294399


  • Relevance
    SPON1 is a member of a subgroup of the thrombospondin type 1 (TSR) class molecules, defined by two domains of homology, the FS1/FS2 and TSR domains. The TSRs of SPON1 proteins are typical of class 2 TSRs. SPON1, which is similar to thrombospondin, is a extracellular matrix attached molecule that promotes neurite outgrowth and inhibits angiogenesis. Analysis of gain and loss of function experiments reveal that SPON1 is required for accurate pathfinding of embryonic axons, and plays a dual role in patterning axonal trajectories. It promotes the outgrowth of commissural and inhibits the outgrowth of motor axons, and has also been implicated in inflammatory processes in the nervous system.
  • Cellular localization
    Secreted protein; extracellular space; extracellular matrix.
  • Database links
  • Alternative names
    • F spondin antibody
    • SPON 1 antibody
    • spondin 1 (f spondin) extracellular matrix protein antibody
    • Spondin 1 antibody
    • Spondin 1 extracellular matrix protein antibody
    • Spondin 1 precursor antibody
    • Spondin1 antibody
    • Vascular smooth muscle cell growth promoting factor antibody
    • VSGP antibody
    • VSGP/F spondin antibody
    see all


  • E coli derived fusion protein as test antigen. Ab14271 dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.

    E coli derived fusion protein as test antigen. Ab14271 dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.


This product has been referenced in:
  • Jiao TT  et al. Importance of spondin 1 and cellular retinoic acid binding protein 1 in the clinical diagnosis of ovarian cancer. Int J Clin Exp Pathol 6:3036-41 (2013). IHC-P ; Human . Read more (PubMed: 24294399) »
  • Clemitson JR  et al. Genetic dissection of a blood pressure quantitative trait locus on rat chromosome 1 and gene expression analysis identifies SPON1 as a novel candidate hypertension gene. Circ Res 100:992-9 (2007). Read more (PubMed: 17332427) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Tissue lysate - whole (Kidney)
Loading amount
20 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%

Dr. Andrew Bingham

Verified customer

Submitted Nov 14 2006

Thank you for taking the time to provide all the protocol details, it was very useful to understand the problem. Could you please tell me the batch number of the first order, we have several records of selling to GeneResearch ab14271 in the last few...

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I'm sorry to hear you are experiencing a problem with ab14271. Thank you for providing so many details about your protocol, I was very useful for me to understand your problem and I am confident that there the antibody is at fault. Unfortunately, I h...

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Thank you for your e-mail. From the Swiss prot information the reelin domain is from aa 29-194 of spondin 1. However the immunogen sequence is located towards the carboxy end of the protein (it is located at aa 549-807 and the whole protein is 807aa l...

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Thank you for your enquiry. Please find the information regarding this protein from this website: http://au.expasy.org/cgi-bin/niceprot.pl?P35446

Thank you for your enquiry. I will attempt to answer your enquiries point by point. 1) Where 20ug is denoted as the loading amount on a WB blot this denotes total protein and not purified target protein. 100ng in the on line protocol refers to the p...

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Thank you for you inquiry. Once an order has been placed for this antibody, it takes approximately 7 to 10 days for it to come into stock. Please contact us again if you have any other questions regarding our products.


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