Overview

  • Product name
  • Description
    Chicken polyclonal to SPON1
  • Host species
    Chicken
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 549-807 of SPON1.

Properties

Applications

Our Abpromise guarantee covers the use of ab14271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 100.9 kDa.
IHC-P Use at an assay dependent concentration. PubMed: 24294399

Target

  • Relevance
    SPON1 is a member of a subgroup of the thrombospondin type 1 (TSR) class molecules, defined by two domains of homology, the FS1/FS2 and TSR domains. The TSRs of SPON1 proteins are typical of class 2 TSRs. SPON1, which is similar to thrombospondin, is a extracellular matrix attached molecule that promotes neurite outgrowth and inhibits angiogenesis. Analysis of gain and loss of function experiments reveal that SPON1 is required for accurate pathfinding of embryonic axons, and plays a dual role in patterning axonal trajectories. It promotes the outgrowth of commissural and inhibits the outgrowth of motor axons, and has also been implicated in inflammatory processes in the nervous system.
  • Cellular localization
    Secreted protein; extracellular space; extracellular matrix.
  • Database links
  • Alternative names
    • F spondin antibody
    • SPON 1 antibody
    • spondin 1 (f spondin) extracellular matrix protein antibody
    • Spondin 1 antibody
    • Spondin 1 extracellular matrix protein antibody
    • Spondin 1 precursor antibody
    • Spondin1 antibody
    • Vascular smooth muscle cell growth promoting factor antibody
    • VSGP antibody
    • VSGP/F spondin antibody
    see all

Images

  • E coli derived fusion protein as test antigen. Ab14271 dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.

    E coli derived fusion protein as test antigen. Ab14271 dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.

References

This product has been referenced in:
  • Jiao TT  et al. Importance of spondin 1 and cellular retinoic acid binding protein 1 in the clinical diagnosis of ovarian cancer. Int J Clin Exp Pathol 6:3036-41 (2013). IHC-P ; Human . Read more (PubMed: 24294399) »
  • Clemitson JR  et al. Genetic dissection of a blood pressure quantitative trait locus on rat chromosome 1 and gene expression analysis identifies SPON1 as a novel candidate hypertension gene. Circ Res 100:992-9 (2007). Read more (PubMed: 17332427) »
See all 2 Publications for this product

Customer reviews and Q&As

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Filter by Species

Filter by Ratings

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Kidney)
Loading amount
20 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%

Dr. Andrew Bingham

Verified customer

Submitted Nov 14 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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